响应面法优化樟芝固态发酵产安卓奎诺尔

以樟芝菌固态发酵生产活性代谢产物安卓奎诺尔为目标物,采用Box-Behnken原理进行响应面分析,对樟芝固态发酵产安卓奎诺尔条件进行优化,结果表明:接种量为296.80 m L/kg、Triton X-100添加量为1.10 m L/kg、辅酶Q0的添加量为0.23 g/kg时,理论上樟芝固态发酵培养基安卓奎诺尔产量的最大值为865.85 mg/kg。经验证,安卓奎诺尔实际产量为865.32 mg/kg,表明实验建立的模型能较好地预测实际发酵产安卓奎诺尔的情况。通过优化,樟芝固态发酵安卓奎诺尔产量比优化前(260.57 mg/kg)提高了232.09%。

Optimization of Antroquinonol Production in Solid-State Fermentation of Antrodia camphorata Using Response Surface Methodology

In this study, response surface methodology based on Box-Behnken design was used to optimize the culture
conditions for the production of the bioactive metabolite antroquinonol in solid-state fermentation of Antrodia camphorata.
The optimal culture conditions were determined as inoculum amount of 296.80 mg/kg, Triton X-100 concentration of
1.10 mL/kg and coenzyme Q0 concentration of 0.23 g/kg. Under these conditions, the maximum predicted yield of
antroquinonol was 865.85 mg/kg, which was in good agreement with the experimental value of 865.32 mg/kg. Therefore, the
established model could be used for predicting the culture conditions of Antrodia camphorate for antroquinonol production.
After optimization, the yield of antroquinonol was increased 232.09% when compared with the control (260.57 mg/kg).