柑橘柠檬苦素大孔树脂分离纯化方法

以柑橘柠檬苦素为考察指标，研究大孔树脂分离纯化柠檬苦素的工艺条件。结果表明，D101大孔树脂对柑橘柠檬苦素有较好的吸附分离性能，是分离纯化柑橘柠檬苦素的适宜大孔树脂；D101大孔树脂分离纯化柑橘柠檬苦素的最佳工艺条件为：上样流速1 mL/min、上样质量浓度0.5 mg/mL、用 pH 6、80%的乙醇溶液作洗脱剂、洗脱流速2 mL/min。通过树脂回收重复使用，发现D101树脂通过再生处理后，其吸附性能未有明显降低，可以重复使用。采用上述方法得到D101大孔树脂对柠檬苦素的吸附率为88.53%，解吸率为93.47%，得率为82.75%。高效液相色谱检测可知，柠檬苦素的含量达到了92.79%。

Separation and Purification of Citrus Limonin by Macroporous Resin

A method for the purification of limonin from citrus by macroporous resin was proposed. The results showed that
D101 resin was the best adsorbent for the purification of citrus limonin. The optimal purification conditions were achieved
by loading the sample containing 0.5 mg/mL limonin onto the column at a flow rate of 1 mL/min and subsequently eluting
the adsorbed limonin with 80% alcohol at pH 6 at a flow rate of 2 mL/min. The adsorption capacity of D101 resin remained
almost unchanged after several cycles of repeated use and regeneration. Under the optimized conditions, the adsorption rate,
desorption rate and yield of limonin were 88.53%, 93.47% and 82.75%, respectively. The result of HPLC showed that the
limonin content (i.e., purity) of the purified product was 92.79%.