蓝靛果花色苷对高脂血症大鼠肝脏内与胆固醇代谢相关基因的作用

目的:研究蓝靛果花色苷对高脂血症大鼠肝脏肝X受体(liver X receptorα,LXRα)、B族I型清道夫受体(scavenger receptor class B,type I,SR-BI)、三磷酸腺苷结合盒转运蛋白G5(ATP-binding cassette transporter G5,ABCG5)、过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptorγ,PPARγ)及胆固醇7α-羟化酶(cholesterol 7α-hydroxylase,CYP7a1)基因表达的影响。方法:选择2月龄雄性Wistar大鼠60只,将大鼠随机分为6组,其中10只给予普通饲料,其余50只给予高脂饲料。30 d造模成功后,分别建立基础饲料正常对照组(ND,灌胃1.2 g/(kg·d)生理盐水)、高脂模型对照组(HFD,灌胃1.2 g/(kg·d)生理盐水)、阳性对照组(灌胃10 mg/(kg·d)辛伐他汀片)和蓝靛果花色苷低(HFD+L)、中(HFD+M)、高(HFD+H)剂量组(分别灌胃4.0、40.0、120.0 mg/(kg·d)蓝靛果花色苷),持续28 d。利用实时荧光定量聚合酶链式反应检测大鼠肝脏组织中LXRα、SR-BI、ABCG5、PPARγ及CYP7a1 mRNA的表达情况。结果:经蓝靛果花色苷干预后,与HFD组相比:蓝靛果花色苷各剂量组肝脏中SR-BI、ABCG5 m RNA表达几乎不受影响,且无显著性差异(P0.05);HFD+M、H F D+H组LXRα、CYP7a1 mRNA表达升高且差异极显著(P0.0 1);H F D+L、HFD+M、HFD+H组PPARγm RNA表达降低且差异极显著(P0.01)。结论:蓝靛果花色苷通过提高高脂血症大鼠肝脏内LXRα、CYP7a1 m RNA表达,降低PPARγ mRNA表达来调节高脂血症大鼠的血脂水平,预防动脉硬化的发生。

Effect of Lonicera caerulea Anthocyan on the Role of Cholesterol Metabolism Related Genes in the Liver of Hyperlipidemic Rats

Objective: The effect of Lonicera caerulea anthocyanin on the expression of liver X receptor α (LXRα), scavenger receptor class B, type I (SR-BI), ATP-binding cassette transporter G5 (ABCG5), peroxisome proliferator-activated receptor γ (PPARγ) and cholesterol 7α-hydroxylase (CYP7a1) in hyperlipidemic rat liver were investigated in this study. Methods: Totally 60 male Wistar rats at the age of two months were randomly divided into 6 groups including 10 rats with normal feed and 50 rats with high-fat diet for the diabetic model establishment. After model establishment in 30 days, the diabetic rats were divided 5 groups with basic diet group (ND, 1.2 g/(kg·d) physiological saline intragastric administration), high-fat diet group (HFD, 1.2 g/(kg·d) physiological saline intragastric administration), 10 mg/(kg·d) simvastatin tablet intragastric administration group as the positive control, and low dose, middle dose and high dose of Lonicera caerulea anthocyanin groups (HFD + L, HFD + M, HFD + H, Lonicera caerulea anthocyanin at 4.0, 40.0 and 120.0 mg/(kg·d). The intragastric administration was last for 28 days. The expression levels of LXRα, SB-BI, ABCG5, PPARγ and CYP7a1 were detected by quantitative real-time polymerase chain reaction (PCR). Results: Compared with HFD group, the mRNA expression levels of SR-BI and ABCG5 mRNA were not significant after the intervention of L. caerulea anthocyanins (P > 0.05); the mRNA expression levels of LXRα and CYP7a1 of HFD + M and HFD + H group in liver tissue were increased (P < 0.05 or P < 0.01); the mRNA expression levels of PPARγ of HFD + L, HFD + M and HFD + H group in liver tissue were decreased. Conclusion: The L. caerulea anthocyan can improve LXRα and CYP7a1 and can decrease PPARγ in liver tissue of hyperlipidemic rats; and regulate blood lipid level of hyperlipidemic rats, thereby preventing arteriosclerosis.