T-2毒素对凡纳滨对虾DNA黏度损伤效应

为探究T-2毒素对凡纳滨对虾DNA黏度的损伤效应，通过小牛胸腺DNA与T-2毒素作用，采用响应面分 析T-2毒素与DNA的最佳作用条件，通过体外T-2毒素与对虾DNA作用验证，以及喂食对虾T-2毒素进行蓄积染毒 后，观察其对DNA黏度的影响。结果：响应面分析优化出对DNA黏度影响最明显的反应条件为T-2毒素质量浓度 2.70 ng/mL、DNA质量浓度50 μg/mL、反应时间43 min；T-2毒素与对虾DNA体外作用，随着T-2毒素质量浓度的增 加，DNA黏度随之增大，在T-2毒素质量浓度为2.70 ng/mL时达到最大值，此后降低；喂食对虾T-2毒素进行蓄积染 毒对对虾DNA黏度的影响无明确规律，与体外对虾DNA实验结果并不相似。本研究阐明T-2毒素对对虾DNA黏度的 影响，对探讨T-2毒素的毒理作用具有一定的意义。

Effect of T-2 Toxin on Viscosity of DNA from Litopenaeus vannamei

The objective of this paper was to explore the effect of T-2 toxin on the viscosity of DNA from shrimps (Litopenaeus vannamei). The conditions for the reaction between calf thymus DNA and T-2 toxin were optimized using response surface methodology. The reaction between shrimp DNA and T-2 toxin in vitro was investigated. Furthermore, the effect of T-2 toxin on DNA viscosity was examined after feeding shrimps with T-2 toxin for 20 days to cause accumulative poisoning. The results obtained showed that a T-2 toxin concentration of 2.70 ng/mL, a DNA concentration of 50 μg/mL and a reaction time of 43 min were determined to be the optimum conditions for the greatest effect of T-2 toxin on DNA viscosity. DNA viscosity rose with increasing concentration of T-2 toxin, reaching its maximum level at 2.70 ng/mL, followed by a decrease. The effect of T-2 toxin accumulation in shrimps on the viscosity of DNA was not clear. This result was dissimilar to the result in vitro. In conclusion, this study may be of great significance for understanding the toxicological effect of T-2 toxin on shrimps.