原花青素B_2对D-半乳糖模型小鼠的代谢组学研究

为探究原花青素B2对D-半乳糖诱导的亚急性衰老模型小鼠的抗衰老作用及特征代谢产物的变化差异,将C57BL/6小鼠随机分为3组:对照组、模型组、原花青素B2组。采用旷场迷宫、Y型食物诱导型迷宫和Morris水迷宫检测各组小鼠的学习记忆能力,并测定小鼠肝、脑、肾中丙二醛(malondialdehyde,MDA)含量、总抗氧化能力(total antioxidant capacity,T-AOC)、超氧化物歧化酶(superoxide dismutase,SOD)活力、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,通过液相色谱-质谱联用技术寻找各组间代谢产物的差异。结果显示:原花青素B2能改善衰老小鼠的学习行为能力,在肝、脑、肾组织中,原花青素B2提高了T-AOC、SOD和GSH-Px活力,而显著降低了MDA的含量(P0.05)。小鼠血浆代谢组学数据显示泛酸、丙酮酸、亚油酸、α-酮戊二酸、马尿酸等17种标志性代谢产物含量发生变化,其抗衰老机制可能涉及糖代谢、脂类代谢等多条代谢通路。

Metabonomics Study on Anti-aging Effect of Procyanidin B2 in D-Galactose-Induced Aging in Mice

In the present study, we investigated the anti-aging effect of procyanidin B2 in D-galactose-induced aging in mice and explored the changes of characteristic metabolites. Four week-old mice were used in this study and randomly divided into normal control group, D-galactose-induced aging group, and procyanidin B2 group. After six weeks of administration, learning and memory capacity in each group were tested by open field maze, Y-maize and Morris water maze and total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content in the brain, liver and kidney of mice were evaluated. The metabolites were analyzed by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The results showed that procyanidin B2 improved learning capacity and it increased significantly T-AOC, SOD and GSH-Px activity, and decreased significantly the content of MDA in liver, brain and kidney tissues when compared with the aging model mice (P < 0.05). Moreover, procyanidin B2 changed the levels of 17 characteristic metabolites, including pantothenic acid, pyruvic acid, linoleic acid, and alpha ketoglutarate, as detected by LC-Q-TOF-MS combined with multivariate statistical analysis. These results indicated that procyanidin B2 was a potential nutrition supplement with anti-aging effect and its underlying mechanism may be associated with a series of metabolism pathways, such as glucose metabolism and lipid metabolism.