| 富硒螺旋藻多肽对血管紧张素转化酶的抑制作用 |
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| 引用本文： | 高冬芳,张逸波,凌钦婕,邹颖,黄峙.富硒螺旋藻多肽对血管紧张素转化酶的抑制作用[J].食品科学,2011(7). |
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| 作者姓名： | 高冬芳 张逸波 凌钦婕 邹颖 黄峙 |
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| 作者单位： | 暨南大学生命科学技术学院; |
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| 基金项目： | 国家自然科学基金项目(20975045;20675045); 暨南大学大学生创新工程项目(cx10088) |
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| 摘 要： | 从富硒螺旋藻(SeSP)中提取总蛋白(SeSP-TP),用不同蛋白酶水解SeSP-TP制备富硒螺旋藻多肽(SeSP-Ps),体外测定SeSP-Ps对血管紧张素转化酶(ACE)的抑制作用。结果表明:5种常见蛋白酶在最适条件下对SeSP-TP水解度明显不同,其中胃蛋白酶-胰蛋白酶-胰凝乳蛋白酶联合使用时的酶水解度最高;虽然不同蛋白酶水解制备的SeSP-Ps均对ACE具有较强的抑制作用,但不同酶水解的SeSP-Ps对ACE的50%抑制浓度(IC50)存在明显差异,其中胃蛋白酶-胰蛋白酶-胰凝乳蛋白酶联合酶解制备的SeSP-Ps对ACE的IC50值最低,为74.6μg/mL。结果提示:SeSP在体内经胃肠蛋白酶消化产生的多肽,对ACE具有较强的抑制活性,且SeSP-Ps对ACE的抑制作用呈现显著的剂量-效应依赖性。
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| 关 键 词： | 血管紧张素转化酶(ACE) 螺旋藻 硒 多肽 |
Angiotensin Convert Enzyme Inhibition of Peptides Derived from Water Soluble Total Protein of Selenium-enriched Spirulina platensis |
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| GAO Dong-fang,ZHANG Yi-bo,LING Qin-jie,ZOU Ying,HUANG Zhi.Angiotensin Convert Enzyme Inhibition of Peptides Derived from Water Soluble Total Protein of Selenium-enriched Spirulina platensis[J].Food Science,2011(7). |
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| Authors: | GAO Dong-fang ZHANG Yi-bo LING Qin-jie ZOU Ying HUANG Zhi |
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| Affiliation: | GAO Dong-fang,ZHANG Yi-bo,LING Qin-jie,ZOU Ying,HUANG Zhi(College of Life Science and Technology,Jinan University,Guangzhou 510632,China) |
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| Abstract: | The water soluble total protein(TP) of selenium-enriched Spirulina platensis(SeSP),named as SeSP-TP,was extracted by phosphate buffer and then hydrolyzed by 5 different proteases to produce SeSP peptides(SeSP-Ps).The angiotensin convert enzyme(ACE) inhibition of SeSP-Ps was measured in vitro.The results showed that treatments with different proteases caused obvious difference of hydrolysis degree(HD) to SeSP-TP,and the combined use of pepsin,trypsin and chimotrypsin led to the highest H.Furthermore,we found... |
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| Keywords: | angiotensin convert enzyme Spirulina platensis selenium peptide |
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