HS-SPME-GC-MS分析新疆胡麻油挥发性成分的技术优化

采用顶空固相微萃取-气相色谱-质谱(headspace solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)联用技术对新疆胡麻油挥发性成分测定分析进行优化,以期获得HS-SPME-GCMS分析胡麻油挥发性香气的最佳条件。结果表明,采用DVB/CAR/PDMS萃取头,在磁力搅拌条件下,胡麻油SPME最佳萃取条件为萃取温度50℃、萃取时间40 min、解吸时间4 min,在该条件下采用HS-SPME-GC-MS鉴定出的胡麻油挥发性物质达到46种,主要包括醛类、酮类、醇类、酸类、酯类、烷烃类、杂环类及其他类物质,其中醛类、杂环类和醇类总相对含量较高,分别为30.14%、12.73%和9.25%,分离鉴定效果较好。

Optimization of Headspace Solid Phase Microextraction (HS-SPME) for Analysis of Volatile Constituents of Flaxseed Oil from Xinjiang by Gas Chromatography-Mass Spectrometry (GC-MS)

This study optimized the headspace solid phase microextraction (HS-SPME) conditions for the analysis of the volatile constituents of flaxseed oil from Xinjiang by gas chromatography-mass spectrometry (GC-MS). The optimum HS-SPME conditions established were as follows: 40 min extraction using a DVB/CAR/PDMS fiber at 50 ℃ with magnetic stirring followed by 4 min desorption. Under these conditions, a total of 46 volatile compounds were identified consisting mainly of aldehydes, ketones, alcohols, acids, esters, alkanes and heterocyclics and other miscellaneous compounds with aldehydes, heterocyclics and alcohols being predominant among these, accounting for 30.14%, 12.73% and 9.25% of the total identified components, respectively. A good chromatographic separation was achieved in this study.