| ICP-MS直接进样对大米酶解液中痕量硒的测定 |
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| 引用本文： | 赵尔敏,方 勇,王明洋,李 彭,胡秋辉,邱伟芬.ICP-MS直接进样对大米酶解液中痕量硒的测定[J].食品科学,2017,38(10):168-172. |
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| 作者姓名： | 赵尔敏 方 勇 王明洋 李 彭 胡秋辉 邱伟芬 |
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| 作者单位： | 1.南京财经大学食品科学与工程学院，江苏省现代粮食流通与安全协同创新中心，
江苏高校粮油质量安全控制及深加工重点实验室，江苏 南京 210023；2.南京农业大学食品科技学院，江苏 南京 210095 |
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| 基金项目： | 国家自然科学基金青年科学基金项目（31101247）；江苏省高校自然科学研究重大项目（13KJA550001）；
江苏高校优势学科建设工程资助项目（PAPD） |
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| 摘 要： | 建立电感耦合等离子体质谱(inductively coupled plasma-mass spectrometry,ICP-MS)直接进样测定大米酶解液中痕量硒的方法,并应用于体外模拟消化实验,得出硒的生物利用率。将样品酶解液离心过膜后ICP-MS直接进样测定硒含量,在0~160μg/L范围内线性相关系数为0.999 5,方法检出限为0.009μg/L,加标回收率在95.3%~102.8%之间,精密度为0.87%~4.48%。ICP-MS直接进样法操作简单环保,可作为大米等粮食酶解液中痕量硒的批量分析方法,并有望用于粮食类补硒食物的营养价值体外初评研究。
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| 关 键 词： | 直接进样 电感耦合等离子体质谱 硒 体外模拟消化 |
Determination of Trace Selenium in Enzymatic Hydrolysates of Rice by Direct Injection-Inductively Coupled
Plasma-Mass Spectrometry |
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| ZHAO Ermin,FANG Yong,WANG Mingyang,LI Peng,HU Qiuhui,QIU Weifen.Determination of Trace Selenium in Enzymatic Hydrolysates of Rice by Direct Injection-Inductively Coupled
Plasma-Mass Spectrometry[J].Food Science,2017,38(10):168-172. |
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| Authors: | ZHAO Ermin FANG Yong WANG Mingyang LI Peng HU Qiuhui QIU Weifen |
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| Affiliation: | 1. Key Laboratory of Grains and Oils Quality Control and Processing of Jiangsu Province, Collaborative Innovation Center for Modern
Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics,
Nanjing 210023, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | A new method was developed for the determination of trace selenium (Se) in enzymatic hydrolysates of rice by
direct injection-inductively coupled plasma-mass spectrometry (ICP-MS), and applied to evaluate the Se bioaccessibility
during in vitro gastrointestinal digestion. After filtration and centrifugation, the prepared samples were determined by direct
injection-ICP-MS. The proposed method showed a good linearity in the concentration range of 0–160 μg/L with correlation
coefficient above 0.999 5 and a limit of detection (LOD) of 0.009 μg/L. The recoveries of spiked samples were between
95.3% and 102.8%, and the relative standard deviations (RSDs) were in the range of 0.87% to 4.48%. Furthermore, the
direct injection-ICP-MS method was successfully applied to determine trace Se in in vitro gastrointestinal digestion products
of cooked rice with short analysis time and environmental friendliness. In conclusion, this method can be used in nutritional
evaluation in vitro of Se-enriched grain materials. |
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| Keywords: | direct injection inductively coupled plasma-mass spectrometry (ICP-MS) selenium in vitro gastrointestinal digestion |
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