| 基于不同识别元件的表面等离子体共振技术在食品安全检测中的研究进展 |
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| 引用本文： | 颜朦朦,佘永新,洪思慧,张 超,曹晓林,王猛强,王珊珊,郑鹭飞,金茂俊,邵 华,金 芬,刘海金,王 静.基于不同识别元件的表面等离子体共振技术在食品安全检测中的研究进展[J].食品科学,2018,39(23):263-271. |
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| 作者姓名： | 颜朦朦 佘永新 洪思慧 张 超 曹晓林 王猛强 王珊珊 郑鹭飞 金茂俊 邵 华 金 芬 刘海金 王 静 |
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| 作者单位： | 1.中国农业科学院农业质量标准与检测技术研究所，北京 100081；2.西藏自治区农畜产品质量安全检验检测中心，西藏 拉萨 850009 |
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| 基金项目： | 国家自然科学基金面上项目（31471654）；中央级公益性科研院所基本科研业务费专项（1610072016009） |
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| 摘 要： | 表面等离子体共振(surface plasmon resonance,SPR)技术传感器具有灵敏度高、无需标记、简单、成本低、可实时监测等优点,已在生命科学、新药研发、食品安全、环境污染检测等方面得到了广泛应用。而识别元件是SPR传感器的重要组成部分,它决定检测的专一性及灵敏度,因此识别元件的研究与发展至关重要。本文分别综述了以抗体、适配体、分子印迹聚合物、蛋白质、肽、酶为识别元件的SPR传感器在食品安全检测中近10年来的研究进展;重点分析了不同识别元件在SPR技术中的特点和优势;提出了今后SPR技术在食品安全检测中的技术难点及新应用前景。
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| 关 键 词： | 分子印迹聚合物 抗体 适配体 酶 识别元件 表面等离子体共振 |
Recent Progress in the Application of Surface Plasmon Resonance Based on Different Recognition Elements in Food Safety Detection |
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| YAN Mengmeng,SHE Yongxin,HONG Sihui,ZHANG Chao,CAO Xiaolin,WANG Mengqiang,WANG Shanshan,ZHENG Lufei,JIN Maojun,SHAO Hua,JIN Fen,LIU Haijin,WANG Jing.Recent Progress in the Application of Surface Plasmon Resonance Based on Different Recognition Elements in Food Safety Detection[J].Food Science,2018,39(23):263-271. |
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| Authors: | YAN Mengmeng SHE Yongxin HONG Sihui ZHANG Chao CAO Xiaolin WANG Mengqiang WANG Shanshan ZHENG Lufei JIN Maojun SHAO Hua JIN Fen LIU Haijin WANG Jing |
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| Affiliation: | 1. Institute of Quality Standard and Testing Technology for Agro-products of Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2. Tibet Testing Center of Quality and Safety for Agricultural and Animal Husbandry Products, Lhasa 850009, China |
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| Abstract: | Surface plasmon resonance (SPR) sensors have found a wide range of applications in various fields such as biological science, pharmaceutical development, food safety detection and environmental pollution detection owing to the advantages of high sensitivity, label-free, simplicity, low cost, and real-time monitoring. Developing recognition elements is of great significance to SPR sensors because they determine the specificity and sensitivity of SPR sensors. Herein, we review the advances made in the last decade in SPR sensors based on various recognition elements such as antibody, aptamer, molecular imprinting polymer, protein, peptide and enzyme for food safety detection. Our focus is on the features and advantages of these recognition elements. Finally, future technical challenges and prospects for the application of SPR technology in food safety detection are presented. |
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| Keywords: | molecular imprinting polymer antibody aptamer enzyme recognition elements surface plasmon resonance |
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