中国野生菰米对大鼠血脂及炎性因子表达的影响

目的：研究中国野生菰米对高脂膳食诱导的胰岛素抵抗大鼠血脂浓度以及炎性因子水平的影响。方法：50 只雄性SD大鼠随机分成阴性对照组，胰岛素抵抗模型组，高、低剂量菰米组和米面组。连续喂养8 周，实验前、后测定各组大鼠的体质量及总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）和高密度脂蛋白胆固醇（high-density lipoprotein cholesterol，HDL-C）浓度，同时测定高敏C反应蛋白（hypersensitive C-reactive protein，hs-CRP）、肿瘤坏死因子-α（tumor necrosis factor，TNF-α）、白介素-6（interleukin-6，IL-6）含量，用SPSS 18.0软件对数据进行统计分析。结果：高剂量菰米组大鼠与米面组及胰岛素抵抗模型组大鼠相比，HDL-C浓度显著上升（P＜0.05），体质量和TC、TG、LDL-C浓度均显著下降（P＜0.05）。与胰岛素抵抗模型组相比，高剂量菰米组的TNF-α及IL-6含量显著下降（P＜0.05）。与阴性对照组相比，低、高剂量菰米组IL-6含量均显著升高（P＜0.05）。结论：中国野生菰米具有改善高脂膳食诱导的大鼠胰岛素抵抗作用，该作用的机制可能是通过降低血脂浓度，调节炎性因子的表达，从而改善胰岛素抵抗。

Effect of Wild Rice Seeds (Zizania latifolia) on Lipid Metabolism and Inflammatory Factor Expression in Rats

Objective: To evaluate the effect of dietary supplementation of wild rice seeds (Zizania latifolia) on blood lipids and inflammatory factor levels in some tissues of high-fat fed rats with insulin resistance. Methods: Totally 50 SD rats were randomly divided into 5 groups: negative control, insulin resistance model, high-dose wild rice, low-dose wild rice and rice flour groups. Before and after these animals were fed for 8 weeks, body mass and total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), hypersensitive C-reactive protein (hs-CRP), tumor necrosis factor (TNF-α), and interleukin-6 (IL-6) levels were measured. The SPSS 18.0 software was used to analyze the experimental data. Results: Compared with the insulin resistance model and rice flour groups, body mass and the levels of TC, TG and LDL-C in rats administered with wild rice at both doses declined significantly (P < 0.05), and the level of HDL-C increased significantly (P < 0.05). Compared with the insulin resistance model group, the level of TNF-α and IL-6 in the high-dose wild rice group significantly decreased (P < 0.05). Both the low-dose and high-dose groups showed a significantly increased level of IL-6 in comparison with the negative control group (P < 0.05). Conclusion: Wild rice seeds have the potential to improve insulin resistance in high-fat diet fed rats, likely by reducing blood lipids and regulating the expression of inflammatory cytokines.