短梗五加果多酚提取工艺优化及抗疲劳作用

目的：短梗五加果多酚的提取工艺及抗疲劳作用探讨。方法：通过正交试验考察提取次数、提取时间及液料比对短梗五加果多酚得率的影响；建立小鼠负重游泳疲劳模型，考察短梗五加果多酚的抗疲劳作用。结果：短梗五加果多酚提取的因素影响次序为：提取时间＞液料比＞提取次数，正交试验结果表明最佳提取工艺条件为液料比20∶1（mL/g）、提取时间60?min、提取2?次，短梗五加果多酚得率为1.426%；体内研究结果表明短梗五加果多酚能显著延长小鼠负重游泳力竭时间，显著增加机体肝糖原、肌糖原含量，明显提高小鼠体内谷胱甘肽过氧化物酶活力，同时降低乳酸和肌酸激酶水平。结论：短梗五加果多酚能提高小鼠抗疲劳能力。

Extraction and Antifatigue Effect of Polyphenols from the Fruits of Acanthopanax sessiliflorus (Rupr. et Maxim.) Seem

Objective: To study the extraction and antifatigue effect of polyphenols from the fruits of Acanthopanax sessiliflorus (Rupr. et Maxim.) Seem. Methods: The effects of number of extraction cycles, extraction time and liquid to solid ratio on the extraction yield of polyphenols were investigated by orthogonal array design. The antifatigue effect was tested using a mouse weight-loaded swimming model. Results: Extraction time was found to be the factor with the greatest influence on the extraction yield of polyphenols followed by liquid to solid ratio and number of extraction cycles. The optimum extraction conditions were found as follows: liquid to solid ratio 20:1 (mL/g), extraction time 60 min, and two extraction cycles. The extraction yield of polyphenols under these conditions was 1.426%. The in vivo results showed that the extracted polyphenols could significantly prolong the exhausting swimming time, increase the levels of liver glycogen and muscle glycogen, enhance glutathione peroxidase activity, and reduced the levels of lactic acid and creatine kinase activity. Conclusion: Polyphenols from the fruits of A. sessiliflorus showed an antifatigue effect in mice.