补料方式对灵芝菌丝体液态深层发酵合成灵芝三萜的影响

在50 L发酵罐中采用4种补料培养方式对灵芝菌丝体液态深层发酵合成灵芝三萜进行比较。结果表明,通过补料可以明显地促进灵芝菌丝体的生长和灵芝三萜的合成,同时,不同的补料方式对菌丝体生长和灵芝三萜合成有不同的影响。采用指数补料方式可获得较高的菌丝体干质量,并提高灵芝三萜含量,与传统的发酵方式相比,采用此补料策略取得了较好的效果。发酵终点灵芝菌丝体干质量达到17.68 g/L,灵芝三萜含量达到4.58 g/100 g干菌丝体,分别比分批发酵提高了65.70%和100.88%。

Effects of Different Feeding Methods on Production of Triterpenes by Ganoderma lucidum in Submerged Fermentation

Four different feeding methods for the production of triterpenes by submerged fed-batch fermentation of Ganoderma lucidum G0119 in a 50 L-fermentor were compared. The results showed that glucose feeding during fermentation could obviously promote the mycelical growth of G. lucidum G0119 and the synthesis of triterpenes, and different feeding methods had different influences on the mycelial dry weight and the concentration of triterpenes in the fermentation broth. The highest mycelical dry weight could be obtained by exponential feeding, as well as high concentration of triterpenes. The results showed that the feeding strategy was the best among all tested ones, giving a mycelial dry weight of 17.68 g/L and a triterpene content of 4.58 g/100 g dry mycelium at the end of fermentation, which were significantly increased by 65.70% and 100.88%, respectively, as compared with fed-batch fermentation.