沙棘果水溶性多糖的分离纯化、组分分析及抗氧化活性的研究

用热水提取沙棘果多糖，乙醇沉淀，并用正交实验确定最佳条件，Sevage法去蛋白，酸性乙醇分级沉淀，分出A、B、C及D四个组分，其中C用DEAE柱层析进一步纯化出C1和C2两个级分，Sephadex G-75及聚丙烯酰胺凝胶电泳证明C1为单一组分，C2为两种多糖混合组分，经薄层层析分析，C1中含葡萄糖。C2的主要级分中含木糖和一未知组分。抗氧化实验表明沙棘多糖具有抗氧化活性，且抗氧化作用随浓度增加而加大。

Study on Isolation, Purification, Analysis of the Components and Anti - Oxidation Activity of the Polysaccharides of the Fruits of Hippophae rhamnoides

The polysaccharides of Hippophae rhamnoides were isolated in hot water,and precipitated with perpendicular experiments.The protein in polysaccharides was removed by Sevage way.The polysaccharides were graded to A.B.C and D by acidic ethanol.C was further purified to C1 and C2 with DEAE column chromatography.Sephadex G-75 column chromatography and polyacrylamide gel electrophoresis provde that C1 was one polysaccharide and C2 was two polysaccharides.Their components were analyzed with thin-layer chromatography and the results were that there was glucose in C1 and there were wylose and other unknown monosaccharide in the main component of C2.The anti-oxidation experiments indicated that the polysaccharides of Hippophae rhamnoides had anti-oxidation activty and this activity was increased with the inereased concentration of the polysaccharides.