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啤酒污染乳酸菌PCR引物的设计与验证
引用本文:陈利娜,王德良,田瑞华. 啤酒污染乳酸菌PCR引物的设计与验证[J]. 酿酒, 2008, 35(1): 57-59
作者姓名:陈利娜  王德良  田瑞华
作者单位:1. 内蒙古农业大学生物工程学院,呼和浩特,010018
2. 中国食品发酵工业研究院,北京,100027
摘    要:根据细菌16SrDNA序列的特点,对啤酒中常见污染乳酸菌16SrDNA序列进行分析,设计合成了针对啤酒污染乳酸菌的特征引物。并用该引物对从啤酒厂分离到的7种乳酸菌进行了检测,PCR结果表明该引物能够准确检测到啤酒中常见的乳酸菌。

关 键 词:啤酒  乳酸菌  16SrDNA  PCR  引物
文章编号:1002-8110(2008)01-0057-03
收稿时间:2007-09-17
修稿时间:2007-09-17

The PCR Primers Design And Identification of Beer Spoilage Lactic Acid Bacteria
CHEN Li-Na,WANG De-Liang,TIAN Rui-Hua. The PCR Primers Design And Identification of Beer Spoilage Lactic Acid Bacteria[J]. Liquor Making, 2008, 35(1): 57-59
Authors:CHEN Li-Na  WANG De-Liang  TIAN Rui-Hua
Affiliation:CHEN Li-Na,WANG De-Liang,TIAN Rui-Hua (1 .Bioengineering College of Inner Mongolia Agricultural University,Huhhot 010018,China; 2.China National Institute of Food and Fermentation Industries,Beijing 100027,China)
Abstract:After studying the characteristic of the bacteria 16S ribosomal RNA gene (16S rDNA) and analyzing their sequences of beer spoilage lactic acid bacteria (LAB), a pair of universal primers were designed for beer spoilage lactic acid bacteria.Using the primers, 7 LABs were identified by polymerase chain reaction (PCR) amplification of the 16S rDNA. It was showed that the primers acted well in accurate identification of LAB in beer by PCR.
Keywords:beer  lactic acid bacteria(LAB)   16s rDNA  polymerase chain reaction (PCR)  primer
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