| 啤酒污染乳酸菌PCR引物的设计与验证 |
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| 引用本文： | 陈利娜,王德良,田瑞华.啤酒污染乳酸菌PCR引物的设计与验证[J].酿酒,2008,35(1):57-59. |
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| 作者姓名： | 陈利娜 王德良 田瑞华 |
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| 作者单位： | 1. 内蒙古农业大学生物工程学院,呼和浩特,010018
2. 中国食品发酵工业研究院,北京,100027 |
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| 摘 要： | 根据细菌16SrDNA序列的特点，对啤酒中常见污染乳酸菌16SrDNA序列进行分析，设计合成了针对啤酒污染乳酸菌的特征引物。并用该引物对从啤酒厂分离到的7种乳酸菌进行了检测，PCR结果表明该引物能够准确检测到啤酒中常见的乳酸菌。
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| 关 键 词： | 啤酒 乳酸菌 16SrDNA PCR 引物 |
| 文章编号： | 1002-8110（2008）01-0057-03 |
| 收稿时间： | 2007-09-17 |
| 修稿时间： | 2007年9月17日 |
The PCR Primers Design And Identification of Beer Spoilage Lactic Acid Bacteria |
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| CHEN Li-Na,WANG De-Liang,TIAN Rui-Hua.The PCR Primers Design And Identification of Beer Spoilage Lactic Acid Bacteria[J].Liquor Making,2008,35(1):57-59. |
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| Authors: | CHEN Li-Na WANG De-Liang TIAN Rui-Hua |
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| Affiliation: | CHEN Li-Na,WANG De-Liang,TIAN Rui-Hua (1 .Bioengineering College of Inner Mongolia Agricultural University,Huhhot 010018,China; 2.China National Institute of Food and Fermentation Industries,Beijing 100027,China) |
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| Abstract: | After studying the characteristic of the bacteria 16S ribosomal RNA gene （16S rDNA） and analyzing their sequences of beer spoilage lactic acid bacteria （LAB）, a pair of universal primers were designed for beer spoilage lactic acid bacteria.Using the primers, 7 LABs were identified by polymerase chain reaction （PCR） amplification of the 16S rDNA. It was showed that the primers acted well in accurate identification of LAB in beer by PCR. |
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| Keywords: | beer lactic acid bacteria（LAB） 16s rDNA polymerase chain reaction （PCR） primer |
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