| 北京棒杆菌高丝氨酸脱氢酶基因的克隆与表达产物活性测定 |
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| 引用本文： | 刘嘉,闽伟红,许金坤,方丽.北京棒杆菌高丝氨酸脱氢酶基因的克隆与表达产物活性测定[J].中国食物与营养,2012,18(6):36-38. |
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| 作者姓名： | 刘嘉 闽伟红 许金坤 方丽 |
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| 作者单位： | 吉林农业大学食品科学与工程学院,长春130118 小麦和玉米深加工国家工程实验室,长春130118 |
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| 摘 要： | 以北京棒杆菌E31染色体DNA为模板,用PCR法扩增了高丝氨酸脱氢酶(HD)基因。将结构基因装载于pET-28a质粒的T7启动子下游,得到了重组表达质粒pET-HD。将其转入大肠杆菌(E.coli)BL21(DE3),经异丙基硫代半乳糖苷(IPTG)诱导,获得了高效的表达。含表达质粒的菌体胞内粗提液经酶活分析表明,高丝氨酸脱氢酶的活性为不含重组质粒的对照菌的10倍。
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| 关 键 词： | 高丝氨酸脱氢酶(HD) PCR 基因表达 |
Clone and Activity Determination of Expression Product of Homoserine Dehydrogenase Gene from Beijing Corynebacterium |
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| LIU Jia,MIN Wei-hong,XU Jin-Kun,FANG Li.Clone and Activity Determination of Expression Product of Homoserine Dehydrogenase Gene from Beijing Corynebacterium[J].Food and Nutrition in China,2012,18(6):36-38. |
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| Authors: | LIU Jia MIN Wei-hong XU Jin-Kun FANG Li |
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| Affiliation: | (;College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China; 2 National Engineering Laboratory on Wheat and Corn Further Processing, Changchun 130118, China) |
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| Abstract: | Based on the DNA sequence of Beijing corynebacterium E31, homoserine dehydrogenase gene was amplified. Structural gene was cloned into downstream of T7 promote and expression plasmid was obtained. The expression plasmid was transferred to E.coli BL21 （DE3）and induced by IPTG, and high expression was obtained. The analysis of enzyme activity indicated that homoserine dehydrogenase of recombinant Escherichia coli BL21 was 10 times as control bacteria. |
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| Keywords: | homoserinedehydrogenase PCR geneexpression |
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