| 生姜蛋白酶的双水相法萃取研究 |
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| 引用本文： | 谢芳,唐晓珍,杜新永,卢会忠.生姜蛋白酶的双水相法萃取研究[J].中国食物与营养,2010(11):50-54. |
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| 作者姓名： | 谢芳 唐晓珍 杜新永 卢会忠 |
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| 作者单位： | [1]山东农业大学食品科学与工程学院,泰安271018 [2]山东省莱芜市莱城区农业局,莱芜271100 |
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| 基金项目： | 山东省科技攻关项目，山东省自然科学基金 |
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| 摘 要： | 以生姜为原料,研究双水相萃取技术分离提取生姜中生姜蛋白酶的提取条件。利用单因素试验与正交试验进行优化,得到最佳萃取方案为PEG分子量为4000,PEG浓度为20%,缓冲盐pH为6.5,缓冲盐浓度为10%。此双水相体系的上相酶活回收和纯化倍数都是最高,分别达279.05%和1.86。
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| 关 键 词： | 双水相萃取 生姜蛋白酶 酶活力 分配系数 |
Study on Aqueous Two-phase System Extraction of Ginger Protease |
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| XIE Fang,TANG Xiao-zhen,DU Xin-yong,LU Hui-zhong.Study on Aqueous Two-phase System Extraction of Ginger Protease[J].Food and Nutrition in China,2010(11):50-54. |
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| Authors: | XIE Fang TANG Xiao-zhen DU Xin-yong LU Hui-zhong |
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| Affiliation: | 1Food Science and Engineering College of Shandong Agricultural University,Taian 271018)(2LaiCheng Section Agriculture Bureau in LaiWu Sity of Shandong,Laiwu 271100) |
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| Abstract: | Ginger as raw material,extraction technology of ginger protease by Aqueous Two-phase System was studied using single factor and orthogonal experiments.Results indicated that the optimum extraction condition was PEG molecular weight 4000,PEG concentration 20%,pH of buffer salt 6.5 and buffer salt concentration 10%.Under above extraction conditions,the GP activity recovery and purification factor were both highest,reached 279.05% and 1.86 respectively. |
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| Keywords: | Aqueous Two-phase System（ATPS） Ginger Protease（GP） GP Activity Ratio |
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