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Doehlert设计优化超声波辅助酶法提取河蚬多糖工艺研究
引用本文:王文骏,廖宁波,刘东红.Doehlert设计优化超声波辅助酶法提取河蚬多糖工艺研究[J].中国食物与营养,2013(12):48-51.
作者姓名:王文骏  廖宁波  刘东红
作者单位:浙江大学生物系统工程与食品科学学院,杭州310058
基金项目:“863”计划(项目编号:2011AA100804).
摘    要:研究用超声波辅助酶法提取河蚬粗多糖,考察超声温度、超声时间、料液比、超声功率对多糖产量的影响,运用Doehlert matrix设计与响应曲面优化得到了最优条件下的最优产量.结果表明,超声波辅助酶法提取河蚬粗多糖最优工艺参数为超声温度82℃、超声时间35min、料液比37mL/g、超声功率270W,多糖产量达到42.7%.

关 键 词:河蚬多糖  超声波辅助酶提取  Doehlert  matrix设计

Application of Doehlert Matrix Design in Optimizing Ultrasonic-assisted Enzymatic Extraction of Polysaccharides from Corbicula fluminea
WANG Wen-jun,LIAO Ning-bo,LIU Dong-hong.Application of Doehlert Matrix Design in Optimizing Ultrasonic-assisted Enzymatic Extraction of Polysaccharides from Corbicula fluminea[J].Food and Nutrition in China,2013(12):48-51.
Authors:WANG Wen-jun  LIAO Ning-bo  LIU Dong-hong
Affiliation:1.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China;)
Abstract:Ultrasonic-assisted Enzymatic Extraction Technology was applied to extract Corbicula fluminea polysaccharides.Four independent variables were extraction temperature,ultrasound time,ratio of material to liquid and ultrasonic power.Doehlert Matrix Design and Response Surface Methodology were used to determine the optimum process parameters that could give highest extraction yield.The optimal polysaccharide yield of 42.7% was obtained under extraction temperature 82℃,ultrasonic time 35min,ratio of material to liquid 37 mL/g and ultrasonic power 270W.
Keywords:Corbicula fluminea polysaccharides  Ultrasonic-assisted Enzymatic Extraction  Doehlert Matrix design
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