| 助朋基因在Bacillus subtilisWB800中的克隆表达 |
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| 引用本文： | 柴海云,崔堂兵.助朋基因在Bacillus subtilisWB800中的克隆表达[J].广州食品工业科技,2012(8):927-929,944. |
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| 作者姓名： | 柴海云 崔堂兵 |
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| 作者单位： | 华南理工大学生物科学与工程学院,广东广州510006 |
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| 基金项目： | 中央高校基本科研业务费项目（20117-110136） |
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| 摘 要： | 对源自Bacillus subtilis168的具有纤溶活性的基因序列（WprA）进行克隆，然后将wpra基因克隆到大肠杆菌．枯草杆菌穿悛载体pBE3中，构建表达载体pBE-WprA，将重组载体转化到Bacillus subtilisWB800中，实现了WprA基因在Bacillus subtilisWB800中的表达。结果表明，WprA基因在Bacillus subtilisWB800中的对数生长期和平衡期均获得了表达，且产物被分泌到胞外。
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| 关 键 词： | Bacillus subtilisWB800 纤溶活性 WprA基因 表达 |
Clonging and Expression of a WprA gene in Bacillus subtilis WB800 |
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| Authors: | CHAI Hai-yun CUI Tang-bing |
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| Affiliation: | (School of Biosciences and Bioengineering, South China University of Technology, Guangzhou 510006, China) |
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| Abstract: | A fibrinolytic enzyme gene （WprA） was cloned fi＇om Bacillus subtilis 168. To efficiently express WprA in Bacillus subtilis WB800, WprA gene was inserted into pBE3 to yield a nove vector pBE-WprA. Then the vector pBE-WprA was transformed and expressed in Bacillus subtilis WB800. Results showed WprA gene was efficiently expressed during the exponential and stationary growth stages, and WprA was secreted into the medium. |
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| Keywords: | Bacillus subtilis WB800 fibrinolytic activity WprA gene expression |
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