| 冰鲜鸭优势腐败菌的鉴定 |
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| 引用本文： | 赵文红,林慧珍,陈海光,张静子,韩珍.冰鲜鸭优势腐败菌的鉴定[J].广州食品工业科技,2012(7):728-732. |
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| 作者姓名： | 赵文红 林慧珍 陈海光 张静子 韩珍 |
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| 作者单位： | [1]仲恺农业工程学院轻工食品学院,广东广州510225 [2]华南农业大学食品学院,广东广州510642 |
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| 基金项目： | 教育部产学研项目（20098090300422）;2009年粤港关键领域重点突破项目（2009A020700005）;广州市重大民生攻关项目（2011YZ-00016） |
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| 摘 要： | 结合传统的细菌分离培养法与现代分子生物技术方法16SrDNA菌群分析法对冰鲜鸭中优势腐败菌进行鉴定。传统分离培养检测到了17株优势腐败菌，16SrDNA菌群分析表明，冰鲜鸭中假单胞菌占绝对优势，达到54％；气单胞茵、肠杆菌是仅次于假单胞菌的第二个类群，都分别占15％；乳酸菌、节杆菌、紫色杆菌以及一株未鉴定的属都分别占4％。
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| 关 键 词： | 冰鲜鸭 腐败菌 传统分离培养 16S rDNA菌群分析法 |
Identification of Spoilage Bacteria in Chilled Duck |
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| Authors: | ZHAO Wen-hong LIN Hui-zhen CHEN Hai-guang ZHANG Jing-zi HAN Zhen |
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| Affiliation: | 1 .College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China) (2.College of Food Science, South China Agriculture University, Guangzhou 510642, China) |
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| Abstract: | Advantage spoilage bacteria were identified in chilled duck by traditional bacteria isolation method and 16S rDNA flora analysis. 17 advantage spoilage bacteria had been detected in chilled duck by traditional bacteria solation method. 16S rDNA flora analysis showed that dominant spoilage bacteria in chilled ducks was of Pseudomonas, being of 54%. Strains of Aeromonas and Enterobacter were the second groups accounted for 15%. The content of a strans ofLactobacillus, Arthrobacter or Bacillus, as well as an unidentified strains, was accounted for 4%. |
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| Keywords: | chilled duck spoilage bacteria traditional bacteria isolation methods 16S rDNA flora analysis |
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