| 葡萄中碳黑曲霉的分离及其产生赭曲霉毒素A的研究 |
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| 引用本文： | 何云龙,梁志宏,许文涛,黄昆仑,罗云波.葡萄中碳黑曲霉的分离及其产生赭曲霉毒素A的研究[J].中外葡萄与葡萄酒,2009(1). |
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| 作者姓名： | 何云龙 梁志宏 许文涛 黄昆仑 罗云波 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 农产品储藏保鲜关键技术研究与示范项目，国家科技基础条件平台项目子课题 |
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| 摘 要： | 碳黑曲霉(Aspergillus carbonarius)是葡萄中产生赭曲霉毒素A(Ochratoxin A,OTA)的重要菌株.采集烟台赤霞珠(Cabernet sauvignon)葡萄,接种于孟加拉红培养基,从中分离到7株黑曲霉群(Aspergillus section black group)真菌,其中3株鉴定属于碳黑曲霉种,占黑色曲霉的43.8%.此3菌株分别接种在粮粒培养基上,静置培养,全部产生OTA,最高浓度达到1300μg/kg,而且,其中2株碳黑曲霉菌株在可可浆培养基上产生荧光,而从葡萄样品中未检出OTA.
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| 关 键 词： | 赤霞珠葡萄 碳黑曲霉 赭曲霉毒素A |
Study on the isolation of Aspergillus carbonarius and ochratoxin A production from wine grape |
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| He Yunlong,Liang Zhihong,Xu Wentao,Huang Kunlun,Luo Yunbo.Study on the isolation of Aspergillus carbonarius and ochratoxin A production from wine grape[J].Sino-Overseas Grapevine & Wine,2009(1). |
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| Authors: | He Yunlong Liang Zhihong Xu Wentao Huang Kunlun Luo Yunbo |
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| Affiliation: | College of Food Science and Nutrition Engineering;China Agricultural University;Beijing 100083 |
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| Abstract: | Aspergillus carbonarius is an important fungus that has been considered to be responsible of the ochratoxin A(OTA) contamination in grape.Healthy grape of Cabernet sauvignon had collected from Yantai vineyard in Shandong province.Samples cultured with Dichloran Rose Bengal Chloramphenicol(DRBC) media and seven fungi belong to Aspergillus section black group were isolated,Thereinto,three were identified as A.carbonarius,and all A.carbonarius were able to produce OTA in grain media,the high concentration was ... |
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| Keywords: | Aspergillus carbonarius Cabernet sauvignon Ochratoxin A |
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