On the monitoring of carotenogenesis by Blakeslea trispora using HPLC |
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Authors: | Fani Mantzouridou Maria Z. Tsimidou |
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Affiliation: | Laboratory of Food Chemistry and Technology, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece |
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Abstract: | A rapid, isocratic RP-HPLC method for the determination of the carotenoids produced by Blakeslea trispora is described. The mixture of acetone:acetonitrile, 60:40, v/v, found appropriate for the cellular triacylglycerol analysis, was also successfully used for the separation of lycopene, γ-carotene and β-carotene. The method was validated for β-carotene using an olive oil triacylglycerol fraction devoid of carotenoids. Recovery study (300 mg/kg oil) was 99%. RSDr and RSDL were satisfactory (<4%). The limit of quantification was found to be 4.56 ng/5 μL and the system was linear in the range 2.0–30.0 ng/μL. |
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Keywords: | Blakeslea trispora Carotenogenesis β-Carotene Intracellular lipids RP-HPLC Quality control |
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