|
|||||
|
|
Monitoring of organophosphorus pesticides in vegetables using monoclonal antibody-based direct competitive ELISA followed by HPLC-MS/MS |
|
| Authors: | Zhen-Lin XuHao Deng Xing-Fei DengJin-Yi Yang Yue-Ming JiangDao-Ping Zeng Fang HuangYu-Dong Shen Hong-Tao LeiHong Wang Yuan-Ming Sun |
| Affiliation: | a Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China b Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China c China National Analytical Center, Guangzhou 510070, China |
| Abstract: | A multi-analytes method for monitoring of organophosphorus pesticides (OPs) using a combination of broad-specificity direct competitive enzyme-linked immunosorbent assay (dcELISA) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The reaction formats of dcELISA as well as the matrix effects of vegetable samples by different treatments were studied. The dcELSIA based on horseradish peroxidase-labelled monoclonal antibody and solid-phase extraction can analyse 42 samples in duplicate simultaneously for 12 OPs with a limit of detection at 20 μg L−1 within 40 min, with good accuracy and reproducibility. For screening purpose, the dcELISA can distinguish positive samples from hundreds of negative samples at a rapid, high-throughput and low cost manner. The positive samples can be following confirmed by HPLC-MS/MS for the kinds and the relative amounts of OPs. The method is suitable for monitoring of OP contamination in vegetables samples with high-efficiency and low cost. |
| Keywords: | Direct competitive ELISA Organophosphorus pesticides Matrix effect Solid-phase extraction HPLC-MS/MS |
| 本文献已被 ScienceDirect 等数据库收录！ | |