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ACE-inhibitory and antihypertensive properties of a bovine casein hydrolysate
Authors:M Miguel  MM Contreras  I Recio  A Aleixandre
Affiliation:1. Instituto de Farmacología y Toxicología (CSIC), Departamento de Farmacología, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain;2. Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain
Abstract:The aim of this study was to investigate the potential angiotensin converting enzyme (ACE)-inhibitory activity and the antihypertensive effect, after a single oral administration, of a pepsin hydrolysed bovine casein (HBC) and a fraction with molecular mass lower than 3000 Da (HBC < 3000). ACE-inhibitory activity was measured by spectrophotometric assay. These products were orally administered by gastric intubation. The systolic (SBP) and the diastolic blood pressure (DBP) were measured in spontaneously hypertensive rats by the tail cuff method before administration and also 2, 4, 6, 8, and 24 h post-administration. HBC showed a potent ACE-inhibitory activity. This activity was 10 times higher in HBC < 3000. HBC and HBC < 3000 decreased the arterial blood pressure of the rats. The decrease in the SBP observed for HBC (400 mg/kg) or HBC < 3000 (200 mg/kg) was less pronounced than that caused by 50 mg/kg of captopril (antihypertensive positive control). However, the maximal decreases in DBP caused by HBC or HBC < 3000 were as high as the maximum decrease observed for captopril. The antihypertensive effect of these products was transient and reverted 24 h after the administration. HBC and HBC < 3000 exert antihypertensive effect caused by small peptides with ACE-inhibitory activity.
Keywords:ACE  angiotensin converting enzyme  BC  bovine casein by enzymatic treatment with pepsin  DBP  diastolic blood pressure  HBC  hydrolysed bovine casein  HBC   <     3000  fraction with molecular mass lower than 3000   Da of HBC  SBP  systolic blood pressure  SHR  spontaneously hypertensive rats
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