Antioxidant activities and contents of polyphenol oxidase substrates from pericarp tissues of litchi fruit |
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| Authors: | Jian Sun Yueming Jiang John Shi Xiaoyi Wei Sophia Jun Xue Jinyu Shi Chun Yi |
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| Affiliation: | 1. South China Botanical Garden, The Chinese Academy of Sciences, 510650 Guangzhou, China;2. Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, 530007 Nanning, China;3. College of Food Science and Biotechnology and Environmental Engineering, Zhejiang Gongshang University, 310035 Hangzhou, China;4. Guelph Food Research Center, Agriculture and Agri-Food Canada, Guelph, Canada N1G 5C9 |
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| Abstract: | The experiments were performed to extract and purify substrates for polyphenol oxidase (PPO) from pericarp tissue of postharvest litchi fruit. Two purified PPO substrates were identified as (−)-epicatechin and procyanidin A2. The antioxidant properties of two PPO substrates were further evaluated in the present study. Variation in the content of the major substrate (−)-epicatechin of litchi fruit during storage at 25 °C was analysed using the HPLC-UV method. The results showed that (−)-epicatechin exhibited stronger antioxidant capability than procyanidin A2, in terms of reducing power and scavenging activities of DPPH radical, hydroxyl radical and superoxide radical. Furthermore, (−)-epicatechin content in pericarp tissue tended to decrease with increasing skin browning index of litchi fruit during storage at 25 °C. Thus, these two compounds can be used as potential antioxidants in litchi waste and the fresh pericarp tissue of litchi fruit exhibited a better utilisation value. |
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| Keywords: | Polyphenol oxidase Litchi Fruits Substrates Content Antioxidant activity |
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