Development of polyclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for sodium saccharin residue in food samples |
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Authors: | Yu Wang Zhen-Lin Xu Yan-Yun Xie Yuan-Xin Tian Yu-Dong Shen Glenn M Young Hong Wang Hong-Tao Lei Yuan-Ming Sun |
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Affiliation: | 1. Key Laboratory of Food Quality and Safety of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou 510642, China;2. School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China;3. Department of Food Science and Technology, University of California, Davis, CA 95616, USA |
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Abstract: | A sensitive and specific polyclonal antibody (PcAb)-based indirect competitive enzyme-linked immunosorbent assay (icELISA) for sodium saccharin is described. 6-Amino saccharin was coupled to carrier protein for artificial antigen by diazotisation. New Zealand white rabbits were immunised to obtain anti-sodium saccharin PcAb and then icELISA was developed. The assay showed high sensitivity and specificity to sodium saccharin, with the 50% inhibition value (IC50) of 0.243 μg mL−1, workable range (IC30–IC70) of 0.050–12.8 μg mL−1 and limit of detection (LOD, IC20) of 0.021 μg mL−1. The average recoveries of sodium saccharin in spiked food samples were estimated ranging from 70.7% to 98.8%. A statistically significant correlation of results was obtained between this new ELISA and previously established HPLC approaches with the food-relevant sodium saccharin concentration range 0–320 μg mL−1 (R2 = 0.9887–0.9975). These results indicated that the established ELISA was a potential and useful analytical tool for rapid determination of sodium saccharin residue in food samples. |
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Keywords: | Sodium saccharin Polyclonal antibody Enzyme-linked immunosorbent assay Artificial sweetener |
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