Optimization of gas chromatography–single quadrupole mass spectrometry conditions for multiresidue analysis of pesticides in grapes in compliance to EU-MRLs |
| |
Authors: | Kaushik Banerjee Sumaiyya Mujawar Sagar C Utture Soma Dasgupta Pandurang G Adsule |
| |
Affiliation: | National Referral Laboratory, National Research Centre for Grapes, P.O. Manjri Farm, Pune 412 307, India |
| |
Abstract: | A single quadrupole GC–MS method was optimized for multiresidue determination of 47 pesticides in grapes with limit of quantifications of each compound in compliance with the EU-MRL requirements. Sample preparation involved extraction of 10 g sample with 10 ml ethyl acetate (+10 g sodium sulphate) by homogenization at 15,000 rpm followed by centrifugation at 3000 rpm. The supernatant was cleaned by dispersive solid phase extraction with primary secondary amine and acidified with 0.1% formic acid. Residues were estimated in selected ion monitoring mode with programmable temperature vaporizer-large volume injection (8 μl). All the GC and MS parameters were thoroughly optimized to achieve satisfactory linearity (R2 > 0.99) within 0.01–0.25 mg kg−1 with minimum matrix interferences. Recoveries at 0.01 and 0.02 mg kg−1 were within 67–120% with associated precision RSD below 19%. The method was successfully applied for analysis of the real world samples for incurred residues. |
| |
Keywords: | Gas chromatography&ndash mass spectrometry Selected ion monitoring Single quadrupole Single laboratory validation Pesticide residues Multiresidue analysis |
本文献已被 ScienceDirect 等数据库收录! |