迷迭香提取物对自由基诱导蛋白质、油脂及DNA氧化的抑制作用

分别采用Cu2+/H2O2、2,2'-偶氮二异丁基脒二盐酸盐2,2'-azobis(2-amidinopropane)hydrochloride,AAPH]诱导牛血清白蛋白(bovine serum albumin,BSA)氧化和羰基化;Fe2+、偶氮二异庚腈2,2'-azobis(2,4-di-methyl-valeronitrile),AMVN]诱导亚油酸(linoleic acid,LA)氧化及AAPH诱导鲱鱼精DNA(herring sperm DNA,hsDNA)氧化损伤,研究迷迭香提取物对蛋白质、油脂以及DNA在自由基攻击下发生氧化损伤的抑制作用。结果表明:25μg/mL^500μg/mL的迷迭香提取物能显著抑制自由基诱导的BSA羰基化和LA氧化产物丙二醛(malondialdehyde,MDA)的生成(p<0.05);100μg/mL^500μg/mL的迷迭香提取物能有效降低AMVN诱导的LA共轭二烯产物的生成;25μg/mL^500μg/mL的迷迭香提取物能显著抑制AAPH诱导的hsDNA氧化产物的生成。结论:迷迭香提取物能够有效抑制自由基诱导的蛋白质、油脂及DNA的氧化,其抑制作用随添加浓度的升高而增强。

Inhibition Effect of Rosemary Extract Against Oxidation of Protein,Lipid and DNA Induced by Free Radicals

Oxidative damage was induced using several different systems to study the inhibition effect of rosemary extract(RE).Oxidation and carbonylation of bovine serum albumin(BSA)was induced by copper ions/hydrogen peroxide and 2,2'-azobis(2-amidinopropane)hydrochloride(AAPH),respectively.Oxidation of linoleic acid(LA)was induced by ferrous iron and 2,2'-azobis(2,4-di-methylvaleronitrile)(AMVN),and oxidative damage to herring sperm DNA(hsDNA)was induced by AAPH.The results showed that 25μg/mL-500μg/mL of RE could significantly inhibit the oxidative cleavage and carbonylation of protein as well as formation of malondialdehyde(MDA)during LA oxidation(p<0.05);and 100μg/mL-500μg/mL of RE could effectively reduce conjugated of LA induced by AMVN.Meanwhile,25μg/mL-500μg/mL of RE could also significantly inhibit hsDNA oxidative cleavage induced by AAPH.It concluded that RE had a strong effect against oxidative damage of protein,lipid,and DNA caused by free radical,and the inhibitory effect is enhanced with the increase of added concentration.