金银花不同溶剂提取物多酚含量及抗氧化活性

分别以水、70%甲醇、70%乙醇和70%丙酮为提取剂,超声辅助提取金银花中多酚,采用Folin-Ciocalteu法测定提取液中多酚量,并利用清除DPPH·、·OH和ABTS+·法评价其抗氧化活性。结果表明,金银花不同溶剂水、70%甲醇、70%乙醇和70%丙酮提取物中多酚含量分别为18.97、46.56、46.63、48.49 mg/g,以水为溶剂与以有机溶液为溶剂,金银花多酚提取量有极显著差异(p<0.01)。不同溶剂金银花多酚均具有一定的清除DPPH·、·OH和ABTS+·能力,即抗氧化活性,但其清除DPPH·和ABTS+·的能力弱于2,6-二叔丁基-4-甲基苯酚(butylated hydroxytoluene,BHT),清除·OH的能力强于BHT。不同溶剂提取的金银花多酚种类不同,对自由基的清除能力不同。

The Content and Antioxidant Activities of Honeysuckle Polyphenol Extracted by Different Solvent

Honeysuckle polyphenols were extracted by ultrasonic-assisted with water,70 % methanol,70 %ethanol,and 70 % acetone as extractant,respectively. The content of polyphenols was determined by Folin-Ciocalteu method,and the antioxidant activities were evaluated by scavenging DPPH·,·OH and ABTS+·. The results showed that the content of polyphenols in water,70 % methanol,70 % ethanol and 70 % acetone extractions were 18.97,46.56,46.63 mg/g and 48.49 mg/g,respectively. The amounts of honeysuckle polyphenols were significantly different between the extraction with water as extractant and the extraction with organic solvent as extractant(p<0.01). Honeysuckle polyphenols from different solvent all had certain ability to scavenge DPPH·,·OH and ABTS+·,namely antioxidant activity. However,the ability to scavenge DPPH·and ABTS+·was weaker than butylated hydroxytoluene(BHT),and the ability to scavenge·OH was stronger than BHT. The types of honeysuckle polyphenols extracted by different solvents were different,and the abilities of scavenging free radicals were different.