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高效液相色谱法检测富硒酵母中的硒代蛋氨酸
引用本文:韦淑毅,钟其顶,高红波,邢江涛,王道兵,熊正河,王岁楼.高效液相色谱法检测富硒酵母中的硒代蛋氨酸[J].食品研究与开发,2012,33(8):164-168.
作者姓名:韦淑毅  钟其顶  高红波  邢江涛  王道兵  熊正河  王岁楼
作者单位:1. 中国药科大学,江苏南京210009;中国食品发酵工业研究院,北京100027
2. 中国食品发酵工业研究院,北京,100027
3. 中国药科大学,江苏南京,210009
摘    要:建立了高效液相色谱法测定富硒酵母中硒代蛋氨酸的分析方法。采用酶解提取法提取富硒酵母样品中的硒代蛋氨酸,丹磺酰氯柱前衍生,C18色谱柱(4.6 mm×250 mm i.d.,5μm)分离,以10 mmol/L磷酸二氢钠缓冲液(含4%N,N-二甲基甲酰胺,pH=6.55)和乙腈为流动相进行梯度洗脱,荧光检测(激发波长:320 nm;发射波长:523nm)。硒代蛋氨酸的检出限为(S/N=3)1μg/L,定量限为(S/N=10)5μg/L。在1 mg/L~50 mg/L范围内的线性关系良好(R≥0.999 6)。加标回收率在91.12%~107.83%,方法相对标准偏差<1.2%。本方法具有专属性好,灵敏度高,适用于富硒酵母原料及相关产品中的硒代蛋氨酸的定量测定。

关 键 词:富硒酵母  硒代蛋氨酸  丹磺酰氯  高效液相色谱法

Determination of Selenomethionine in Selenium-enriched Yeast by Reversed Phase High Performance Liquid Chromatography
WEI Shu-yi , ZHONG Qi-ding , GAO Hong-bo , XING Jiang-tao , WANG Dao-bing , XIONG Zheng-he , WANG Sui-lou.Determination of Selenomethionine in Selenium-enriched Yeast by Reversed Phase High Performance Liquid Chromatography[J].Food Research and Developent,2012,33(8):164-168.
Authors:WEI Shu-yi  ZHONG Qi-ding  GAO Hong-bo  XING Jiang-tao  WANG Dao-bing  XIONG Zheng-he  WANG Sui-lou
Affiliation:1,(1.China Pharmacutical University,Nanjing 210009,Jiangsu,China;2.National Research Institute of Food & Fermentation Industries,Beijing 100027,China)
Abstract:The high performance liquid chromatography detection methods for selenomethionine in selenium-enriched yeast is established,which utilizes protein enzymes for selenomethionine extraction,dansyl chloride for derivatizaiton,C18 reverse column(4.6 mm×250 mm i.d.,5 μm) for seperation,10 mmol/L phosphate buffer solution and acetonitrile for gradient elution,fluorescent detector(λex: 320 nm;λem: 523 nm) for determination.The detection limit(S/N=3) is 1 μg/L,and quantification limit(S/N=10) is 5 μg/L.Detection of 1 to 50 mg/L selenomethionine produces a linear response(R≥0.999 6) and the recovery is quantitative around 91.12 %-107.83 %,the RSD<1.2 %.The present methods is speficitive,sensitive,economatic and accurate,and be used in the determination of selenomethionine for the selenium-enriched yeasts materials and relating products.
Keywords:selenomethionine  selenium-enriched yeast  dansyl chloride  hige performance liquid chromatography
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