菌酶协同发酵水解大米蛋白ACE抑制肽及其活性的研究

使用植物乳杆菌2-18和枯草芽孢杆菌Y、枯草芽孢杆菌Y4-2联合蛋白酶共同水解大米蛋白,测定大米蛋白的水解溶出率及脱除液的血管紧张素转换酶(angiotensin I-converting enzyme,ACE)抑制活性。在利用菌酶联合水解大米蛋白的组合中,植物乳杆菌2-18+风味蛋白酶/菠萝蛋白酶+胃蛋白酶/酸性蛋白酶组合对大米蛋白的水解溶出率最高,蛋白脱除率为(91.32±1.60)%。大米蛋白水解溶出液中必需氨基酸占总氨基酸含量的39.62%。通过对分子量分布分析,大米蛋白水解溶出液的多肽分子量分布主要在1 kDa～1.5 kDa部分。经植物乳杆菌2-18+风味蛋白酶/菠萝蛋白酶+胃蛋白酶/酸性蛋白酶组合对大米蛋白的水解溶出液ACE抑制率达(91.95±1.63)%,具有良好的ACE抑制活性。

Lactobacillus plantarum and Bacillus subtilis Coupled with Enzymes to Remove Rice Protein and the Angiotensin I-Converting Enzyme Inhibitory Activities of Removal Liquid

The rice protein was removed by the combination of Lactobacillus plantarum 2-18 and Bacillus subtilis Y, Bacillus subtilis Y4-2,and the rice protein removal rate and the angiotensin I-converting enzyme(ACE)inhibitory activity of rice protein removal solution were determined.In the groups of using the combination of bacterium and enzyme to remove the rice protein,the combination of Lactobacillus plantarum 2-18+ Flavor protease/bromelain+pepsin/acid protease had the highest removal rate of rice protein,which was(91.32±1.60)%.The essential amino acids in the rice protein removal solution accounted for 39.62 % of the total amino acid content.Through molecular weight distribution analysis,the molecular weight distribution of the peptide of the rice protein removal solution is mainly in the 1 kDa -1.5 kDa portion.The ACE inhibition rate of the protein removal solution obtained by Lactobacillus plantarum 2-18+ flavor protease/bromelain + pepsin/acid protease combined with rice protein was(91.95±1.63)%,and its removal solution has well ACE inhibitory activity.