| 山楂叶总黄酮的水浸提和纯化工艺研究 |
| |
| 引用本文： | 张黎明,杨鑫,曹宇,高文远.山楂叶总黄酮的水浸提和纯化工艺研究[J].食品研究与开发,2010,31(1). |
| |
| 作者姓名： | 张黎明 杨鑫 曹宇 高文远 |
| |
| 作者单位： | 1. 天津科技大学生物工程学院,天津市工业微生物重点实验室,天津,300457
2. 天津大学药物科学与技术学院,天津,300072 |
| |
| 摘 要： | 研究山楂叶总黄酮的水浸提和大孔吸附树脂纯化耦合工艺.重点探讨采用水浸提法提取山楂叶总黄酮的最佳工艺条件.试验结果表明:山楂叶黄酮类化合物的最优水提工艺参数是料液比为1:20(g/mL).浸提时间为120 min,提取次数2次.在此条件下进行回流提取,山楂叶总黄酮的提取率达到87.2%.采用水浸提法与D101型大孔吸附树脂耦合时山楂叶总黄酮进行吸附纯化.该树脂对总黄酮的动态吸附率大于80%,稳定性试验重复次数为5,以体积分数70%乙醇为洗脱剂时解吸率大于86%.山楂叶总黄酮经上述工艺分离纯化后,得率为1.9%,纯度为82.8%.
|
| 关 键 词： | 山楂叶 黄酮类化合物 水浸提 大孔吸附树脂 纯化 |
Water-extracting and Purifying Technology on Flavonoids from Hawthorn Leaves |
| |
| ZHANG Li-ming,YANG Xin,CAO Yu,GAO Wen-yuan.Water-extracting and Purifying Technology on Flavonoids from Hawthorn Leaves[J].Food Research and Developent,2010,31(1). |
| |
| Authors: | ZHANG Li-ming YANG Xin CAO Yu GAO Wen-yuan |
| |
| Affiliation: | ZHANG Li-ming1,YANG Xin1,CAO Yu1,GAO Wen-yuan2 (1.Tianjin Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science , Technology,Tianjin 300457,China,2.College of Pharmaceutical Science , Technology,Tianjin University,Tianjin 300072,China) |
| |
| Abstract: | A technology for water-extracting and purifying flavonoids from hawthorn leaves with macroporous resin was studied.By means of water-extracting method,the optimum extraction conditions of flavonoids in hawthorn leaves were determined.The results showed that the optimum conditions of water-extracting were obtained as follows:the ratio of material to solvent is 1:20(g/mL),it is decocted 2 times and each time is sustained for 120 min.On the basis of these experiments,the average extraction rate of total flavon... |
| |
| Keywords: | hawthorn leaves flavonoids water-extracting macroporous resin purification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
