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DNS法测定玉米秸秆中总糖
引用本文:孙伟伟,曹维强,王静.DNS法测定玉米秸秆中总糖[J].食品研究与开发,2006,27(6):120-123.
作者姓名:孙伟伟  曹维强  王静
作者单位:1. 东北农业大学食品学院,黑龙江,哈尔滨,150030
2. 惠州出入境检验检疫局,广东,惠州,516001
3. 中国农业科学院质标所,北京,100081
摘    要:对DNS法测定玉米秸秆中总糖的各影响因素进行了深入探讨,确定最佳条件:检测波长530nm,显色剂用量1.5mL,沸水浴中保持5min。当水解总糖所用盐酸浓度为6mol/L时,所测总糖主要包括淀粉多糖及寡糖等所水解的还原糖,不包括纤维素等水解的还原糖。

关 键 词:玉米秸秆  DNS法  总糖
收稿时间:2006-04-17
修稿时间:2006年4月17日

COLORIMETRIC DETERMINATION OF WATER-SOLUBLE TOTAL SUGAR IN CORN STALK WITH 3,5-DINITROSALICYLIC ACID
SUN Wei-wei,CAO Wei-qiang,WANG Jing.COLORIMETRIC DETERMINATION OF WATER-SOLUBLE TOTAL SUGAR IN CORN STALK WITH 3,5-DINITROSALICYLIC ACID[J].Food Research and Developent,2006,27(6):120-123.
Authors:SUN Wei-wei  CAO Wei-qiang  WANG Jing
Institution:1.Food Science College, Northeast Agriculture University, Harbin 150030, Heilongjiang, China; 2.Huizhou Entry-Exit Inspection and Quarantine Bureau, Huizhou 516001, Guangdong, China;3. The Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract:The experiment studied on the optimum conditions for 3,5-Dinitrosalicylic acid colorimitry. We discussed the wavelength, dosage of chromogenic, and coloring time, which have effect on the result. According to the experiment, the above factors were determined as 530 nm, 1.5 mL and 5 min respectively. The total sugar is mainly water-soluble total sugar as the concentration of hydrochloric acid is at 6 mol/L.
Keywords:corn stalk  3  5-Dinitrosalicylic acid colorimitry  water-soluble total sugar
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