分散固相萃取净化UPLC测定烟草中4种水溶性糖 |
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引用本文: | 黄淼,杨敬国,姬厚伟,惠非琼,王芳,庞红蕊,黄锡娟,刘剑.分散固相萃取净化UPLC测定烟草中4种水溶性糖[J].中国烟草学报,2018,24(4):16-19. |
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作者姓名: | 黄淼 杨敬国 姬厚伟 惠非琼 王芳 庞红蕊 黄锡娟 刘剑 |
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作者单位: | 贵州中烟工业有限责任公司技术中心, 贵阳市小河区开发大道96号 550009 |
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基金项目: | 贵州中烟工业有限责任公司科技项目“贵烟品类卷烟风格特征的化学物质基础研究”黔烟工技[2015]8号 |
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摘 要: | 建立了分散固相萃取净化-超高效液相色谱/蒸发光散射检测器(UPLC/ELSD)快速测定烟草中4种水溶性糖(果糖、葡萄糖、蔗糖、麦芽糖)含量的方法。在优化的超高效液相色谱条件下,10分钟内可以实现4种糖的出峰及分离并快速完成定量检测。该方法具有快速、简单、灵敏等特点,应用于实际烟草样品检测取得了良好效果。
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关 键 词: | 分散固相萃取 UPLC 烟草 水溶性糖 |
收稿时间: | 2017-09-06 |
Determination of four water-soluble sugars in tobacco by dispersive solid-phase extraction coupled with ultra high performance liquid chromatography |
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Affiliation: | Technology Center, China Tobacco Guizhou Industrial Co.Ltd, Guiyang 550009, China |
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Abstract: | An ultra high performance liquid chromatography (UPLC) method coupled with evaporative light scattering detector (ELSD)and dispersive solid-phase extraction (DSPE) was successfully developed.Using this method, the contents of four water-soluble sugars(fructose, glucose, sucrose, maltose) in tobacco were rapidly determined within 10 min.Results show that the method is rapid, simple and sensitive for the determination of water-soluble sugar content in tobacco. |
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