土壤中降解烟碱细菌多样性研究

为了解烟草根围土壤中可降解烟碱细菌的多样性,采用0.1％的烟碱为唯一碳源和氮源的无机盐培养基,从云南省普洱、玉溪、曲靖、楚雄、大理五个地区59个土样中分离得到302株降解烟碱细菌;16S rDNA序列分析结果表明,302株菌分属厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、变形菌 门(Proteobacteria)和拟杆菌门(Bacteroidetes)四大类群,分别与数据库中已知的节杆菌属(Arthrobacter)、芽孢杆菌属(Bacillus)、类芽孢杆菌属(Paenibacillus)、微球菌属(Micrococcus)、纤维单胞菌属(Cellulomonas)、假单胞菌属(Pseudomonas)、短状杆菌属(Brachybacterium)、根瘤菌属(Rhizobium)、中华根瘤菌属(Sinorhizobium)、土壤杆菌属(Agrobacterium)、不动杆菌属(Acinetobacter)、剑菌属(Ensifer)、两面神菌属(Janibacter)、鞘脂杆菌属(Sphingobacterium)、Balneimonas具有较高的相似性.其中节杆菌属、芽孢杆菌属和类芽孢杆菌属为土壤中降解烟碱细菌的优势菌属.

Analysis of diversity of nicotine-degrading bacteria isolated from soil

302 bacteria strains were isolated by mineral salt medium supplemented with 0.1% nicotine as sole carbon and nitrogen sources from 59 soil samples from Puer,Yuxi,Qujing,Chuxiong and Dali districts in Yunnan province.Based on sequence analysis of 16S rDNA,302 strains were grouped into four phyla,i.e.,Firmicutes,Actinobacteria,Proteobacteria and Bacteroidetes,and were further clustered into 15 genera:Arthrobacter,Bacillus,Paenibacillus,Micrococcus,Cellulomonas,Pseudomonas,Brachybacterium,Rhizobium,Sinorhizobium,Agrobacterium,Acinetobacter,Ensifer,Janibacter,Sphingobacterium and Balneimonas.Results showed that Arthrobacter,Bacillus and Paenibacillus were the dominant nicotine-degrading bacteria groups.