| 烟草黑胫病菌全基因组DNA遗传多态性RAPD标记 |
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| 引用本文： | 张修国,罗文富,吴德喜,张世光,尚志强.烟草黑胫病菌全基因组DNA遗传多态性RAPD标记[J].中国烟草学报,1999(2). |
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| 作者姓名： | 张修国 罗文富 吴德喜 张世光 尚志强 |
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| 作者单位： | 云南农业大学云南省植物病理重点实验室，云南省玉溪烟科所 |
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| 摘 要： | 应用ＲＡＰＤ分子标记技术，从８０个随机引物中筛选出１０个分别为ＯＰＡ０２、ＯＰＡ０１３、ＯＰＡ０１４、ＯＰＡ０１６、ＯＰＡ０１８、ＯＰＢ０４、ＯＰＢ０５、ＯＰＤ０２、ＯＰＤ０３、ＯＰＤ０１１，对来自云南省６大主产烟区的２４个烟草黑胫病菌株全基因组ＤＮＡ进行随机扩增。结果表明：１０个引物共标记出８９条ＲＡＰＤ图带，其中多态性图带６３条，多态率７０．７％。引物ＯＰＤ０１１、ＯＰＤ０２、ＯＰＡ０１６、ＯＰＢ０４对每个受试样品均可标记出分子量为０．８７Ｋｂ和１．１Ｋｂ的ＤＮＡ片段，此４个引物对受试样品全基因组ＤＮＡ具有特征性分子标记的特性，利用核酸分子杂交技术对田间寄主体内的黑胫病菌的有无进行分子检测。被标记出的分子量为０．８７Ｋｂ和１．１Ｋｂ的特征性ＤＮＡ片段，可用来制备特异性探针，对田间烟草黑胫病菌发生动态进行检测和预报。
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| 关 键 词： | 烟草黑胫病菌 遗传多态性 RAPD |
Investigation into the Genetic Divergence of P.Parasitica var. Nicotiane by RAPD Markers |
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| Zhang Xiuguo Luo Wenfu Wu Dexi Zhang Shiguang Shang Zhiqiang.Investigation into the Genetic Divergence of P.Parasitica var. Nicotiane by RAPD Markers[J].Acta Tabacaria Sinica,1999(2). |
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| Authors: | Zhang Xiuguo Luo Wenfu Wu Dexi Zhang Shiguang Shang Zhiqiang |
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| Affiliation: | Zhang Xiuguo 1Luo Wenfu 1Wu Dexi 1Zhang Shiguang 1Shang Zhiqiang 21Department of Pathology,Yunnan Agricultural University,Yunnan 6502012Yuxi Tobacco Research Institute,Yunnan 650212 |
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| Abstract: | Random amplified polymorphic DNA(RAPD)analysis on 24 strains of P.Parasitica var. Nicotiane Tucker which were derived from 6 different areas of Yunnan Province were conducted with 10 effective primers selected from 80 random primers. About 70.7% of the eightynine bands shown polymorphism.Two characteristic RAPD bands(0.87Kb,1.1Kb)were amplified by OPD011,OPD02,OPD04,OPD016 and used as molecular markers to identify the DNA of P.Parasitica var. Nicotiane Tucker.Results show that the characteristic RAPD bands can be used to make specific probe for field inspection of the pathogen. |
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| Keywords: | P Parasitica var Nicotiane RAPDGenetic divergence |
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