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| 毛细管气相色谱法测定烟草中葡萄糖、果糖、蔗糖的含量 | |
| 引用本文： | 张峻松,宣晓泉,唐纲岭,徐如彦,王建民.毛细管气相色谱法测定烟草中葡萄糖、果糖、蔗糖的含量[J].中国烟草学报,2007,13(2):17-20. |
| 作者姓名： | 张峻松 宣晓泉 唐纲岭 徐如彦 王建民 |
| 作者单位： | 1. 郑州轻工业学院,郑州东风路5号,450002 2. 徐州卷烟厂,徐州,221005 3. 郑州烟草研究院,郑州,450001 |
| 基金项目： | 郑州轻工业学院校科研和教改项目 |
| 摘 要： | 建立了一种用毛细管气相色谱法测定不同烟草样品中水溶性葡萄糖、果糖、蔗糖含量的方法。以80%(v/v)乙醇提取烟样中水溶性糖,糖液利用三甲基氯硅烷进行硅醚化反应后,进行毛细管气相色谱分析。结果表明葡萄糖、果糖和蔗糖的含量与其峰面积具良好的线性(相关系数在0.9995~0.9998),重复性好(RSD<1.39%),加标回收率在96.19%~99.16%,可以实现对烟草中单糖的分离鉴定。 |
| 关 键 词： | 烟草 葡萄糖 果糖 蔗糖 气相色谱 |
| 文章编号： | 24370314 |
| 修稿时间： | 10 8 2006 12:00AM |
Analysis of glucose,fructose and sucrose in tobacco by capillary gas chromatography |
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| ZHANG Jun-song,XUAN Xiao-quan,TANG Gang-ling,XU Ru-yan,WANG Jian-min.Analysis of glucose,fructose and sucrose in tobacco by capillary gas chromatography[J].Acta Tabacaria Sinica,2007,13(2):17-20. | |
| Authors: | ZHANG Jun-song XUAN Xiao-quan TANG Gang-ling XU Ru-yan WANG Jian-min |
| Affiliation: | 1 Zhengzhou Institute of Light Industry, Zhengzhou 450002, China ; 2 Xuzhou Cigarette Factory, Xuzhou 221005,China; 3 Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001,China |
| Abstract: | A novel method is proposed for the quantitative determination of water-soluble sugars such as glucose, fructose, and sucrose in tobacco. It included extraction of sugars with 80% (v/v) ethanol, trimethylchlorosilane reaction, and quantitative analysis of capillary gas chromatography. The method shows good linearity with correlation coefficients between 0.9995 and 0.9998,and the average recoveries were 99.16%, 97.98%, and 96.19% with good repeatability for glucose, fructose, and sucrose, respectively. |
| Keywords: | tobacco glucose fructose sucrose gas chromatography |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |