邵武烟田土壤微生物群落结构变化与烟草青枯病发生关系初报

为了研究健康和发病烟田土壤微生物群落结构的差异，分别在烟株不同生长时期采取健康和发病烟田土壤，对土壤微生物进行16S rRNA和18S rRNA基因测序，分析其群落结构和多样性的变化。结果表明:与移栽前相比，烟株旺长期土壤微生物群落结构已发生了较大变化，旺长期土壤中酸杆菌门（Acidobacteria）相对丰度平均减少了5.4%，放线菌门（Actinobacteria）增多了8.0%，子囊菌门（Ascomycota）增多了22.8%；健康土壤中unclassifed_f__Micrococcaceae相对丰度是发病土壤的2倍，而发病土壤中假霉样真菌属（Pseudallescheria）相对丰度是健康土壤的2倍；健康土壤微生物多样性较高于发病土壤。微生物群落结构在健康和发病土壤之间存在较大差异，unclassifed_f__Micrococcaceae、假霉样真菌属（Pseudallescheria）等土壤微生物是造成差异的主要物种。 

A preliminary report on relationship between variation of microbial community structure in soil and occurrence of tobacco bacterial wilt in tobacco field in Shaowu

Tobacco bacterial wilt in southern China can cause big economic losses. To understand the differences of soil microbial community structure between healthy and diseased soils and provide theoretical basis for the control of tobacco bacterial wilt, soil samples from healthy and diseased tobacco felds at different growth stages of tobacco plants were collected as research objects, and 16S rRNA and 18S rRNA genes were sequenced to analyze the changes in community structure and diversity of soil microorganisms. Results showed that the microbial community structure of soil in vigorious growth period had changed greatly compared with that before transplanting. The relative abundance of Actinobacteria decreased by an average of 5.4%, Actinobacteria increased by 8.0%, and Ascomycota increased by 22.8%. The relative abundance of unclassifed_f__Micrococcaceae in healthy soil was twice as high as that in infected soils, while the relative abundance of Pseudallescheria in diseased soil was twice that of healthy soil. The soil microbial diversity of healthy soil was higher than that of diseased soil. In conclusion, the microbial community structure differs between healthy and diseased soil, soil microorganisms such as unclassifed_f__Micrococcaceae, Pseudomycetes were the main species causing these differences.