| Abstract: | The trypsin inhibitor fraction from black gram was isolated by ammonium sulphate fractionation (0-75%). It exerted maximum inhibition on trypsin and to a less extent on the endopeptidases, chymotrypsin, papain, pronase and pepsin in vitro, In-vitro digestibility of the raw black gram was found to be lower than autoclaved black gram and autoclaved black gram supplemented with native black gram trypsin inhibitor. Haemagglutinating activity was absent in the black gram extract as well as in the black gram trypsin inhibitor fractions. During the last 48 h of germination there was a steep decline in trypsin inhibitory activity and an increase was observed later. Trypsin inhibitory activity was greater in the leaves than the cotyledons during the fourth, fifth and sixth days of germination. |
