一株耐高浓度乙醇酒精酵母的筛选及发酵特性的研究

为获得乙醇耐受性较高的酵母菌,通过富集培养,从白酒窖池的酒糟中分离筛选出一株耐18%vol乙醇浓度的菌株A2。其最适生长温度为32℃,最适pH值为4.5。通过正交试验得出A2的最优发酵条件为葡萄糖浓度20%(w/v),温度34℃,pH值为4.5。发酵72h后,发酵液酒精浓度达到9.5%vol。

Screening of a high alcohol tolerant yeast and study on its fermentation performance

To isolate high alcohol tolerant yeast,enrichment culture was used to separate a yeast named A2 from grain stillage of Chinese liquor,which could tolerant the concentration of alcohol at 18%vol.The optimum growth temperature and pH of A2 were 32℃ and 4.5,respectively.The optimum fermentation conditions of A2 were obtained by orthogonal experiments as follows： glucose 20%（w/v）,fermentation 34℃ and initial pH 4.5.After 72h of fermentation,the yield of alcohol reached 9.5%vol.