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| 响应面法优化里氏木霉Rut C-30产纤维素酶液体培养基 | |
| 引用本文： | 李勇昊,姜永生,周长海,顾芮萌,孙文良,田朝光.响应面法优化里氏木霉Rut C-30产纤维素酶液体培养基[J].中国酿造,2012,31(4):29-32. |
| 作者姓名： | 李勇昊 姜永生 周长海 顾芮萌 孙文良 田朝光 |
| 作者单位： | 1. 中国科学院天津工业生物技术研究所,天津300308;吉林大学生物与农业工程学院,吉林长春130022 2. 中国科学院天津工业生物技术研究所,天津300308;内蒙古工业大学化工学院,内蒙古呼和浩特010051 3. 吉林大学生物与农业工程学院,吉林长春,130022 4. 中国科学院天津工业生物技术研究所,天津,300308 |
| 基金项目： | 中国科学院百人计划和院方向性项目 |
| 摘 要： | 该试验在单因素对里氏木霉(Trichoderma reesei)Rut C-30产纤维素酶的液体培养基优化的基础上,以滤纸酶活为响应值,采用响应面法确定其最佳培养基。首先通过Plackett-Burman(PB)设计筛选出影响滤纸酶活的显著因素,结晶纤维素和麸皮;通过最陡爬坡试验逼近最大酶活力区域;最后通过Central Composite Design(CCD)设计及响应面分析确定产酶最佳培养基,其中影响酶活的显著性因素结晶纤维素41.8g,麸皮19.1g。经过优化,滤纸酶活力最高为8.21U/mL,比单因素优化结果7.03U/mL提高了16.78%,同时测得CMC酶活为63.64IU/mL,木聚糖酶活为27.4IU/mL,葡萄糖苷酶酶活0.96IU/mL。 |
| 关 键 词： | 纤维素酶 里氏木霉RutC-30 液体发酵 响应面法 滤纸酶活 |
Optimization of liquid-state fermentation medium for cellulase production by Trichoderma reesei Rut C-30 with response surface methodology |
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| LI Yonghao , JIANG Yongsheng , ZHOU Changhai , GU Ruimeng , SUN Wenliang , TIAN Chaoguang.Optimization of liquid-state fermentation medium for cellulase production by Trichoderma reesei Rut C-30 with response surface methodology[J].China Brewing,2012,31(4):29-32. | |
| Authors: | LI Yonghao JIANG Yongsheng ZHOU Changhai GU Ruimeng SUN Wenliang TIAN Chaoguang |
| Affiliation: | 1.Tianjin Institute of Industrial Biotechnology,CAS,Tianjin 300308,China;2.School of Biological and Agricultural Engineering, Jilin University,Changchun 130022,China;3.College of Chemical Engineering,Inner Mongolia University Technology, Hohhot 010051,China) |
| Abstract: | Using filter paper activity（FPA） as response values,response surface methodology was used to optimize liquid-state fermentation medium for cellulase production by Trichoderma reesei Rut C-30.Plackett-Burman design was further adopted to screen the important factors affecting FPA,which were Avicel and wheat bran.The steepest ascent experiment was used to approach the optimal region of the highest FPA.The optimal fermentation medium obtained by Central Composite Design and response surface analysis was as follows： Avicel 41.8g/L,wheat bran 19.1g/L.Based on the optimized medium,FPA reached 8.21U/mL,which was increased by 16.78% when compared with that（7.03IU/ml） before optimization. |
| Keywords: | cellulases Trichoderma reesei Rut C-30 liquid-state fermentation response surface methodology filter paper activity |
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