|
|||||
|
|
| 黑曲霉M2固态发酵产液化酶和糖化酶的研究 | |
| 引用本文： | 吴荣荣,张志民,张煜行,佟兰欣,安惠玲,陈建春,肖冬光.黑曲霉M2固态发酵产液化酶和糖化酶的研究[J].中国酿造,2012,31(1):107-110. |
| 作者姓名： | 吴荣荣 张志民 张煜行 佟兰欣 安惠玲 陈建春 肖冬光 |
| 作者单位： | 1. 天津科技大学生物工程学院,天津300222;河北衡水老白干酿酒(集团),河北衡水053000;衡水学院生命科学学院,河北衡水053000 2. 河北衡水老白干酿酒(集团),河北衡水,053000 3. 天津科技大学生物工程学院,天津,300222 |
| 基金项目： | 衡水市科技支撑计划项目 |
| 摘 要： | 本研究对黑曲霉M2菌株固态发酵产液化酶和糖化酶特性进行了分析,在单因素试验基础上,采用正交试验对发酵条件进行了探讨,菌株M2液化酶固态发酵的最佳工艺参数为温度27℃,接种量3×106孢子/g干基,培养时间4.5d和水分含量34%,其含量达到7.65U/g;糖化酶固态发酵最佳参数温度28℃,接种量3×106孢子/g干基,培养时间3.5d和水分含量35%,其含量为630U/g。 |
| 关 键 词： | 黑曲霉 液化酶 糖化 固态发酵 |
Production of liquifying enzyme and glucoamylase by Aspergilus niger M2 in solid-state fermentation |
|
| WU Rongrong , ZHANG Zhimin , ZHANG Yuhang , TONG Lanxin , AN Huiling , CHEN Jianchun , XIAO Dongguang.Production of liquifying enzyme and glucoamylase by Aspergilus niger M2 in solid-state fermentation[J].China Brewing,2012,31(1):107-110. | |
| Authors: | WU Rongrong ZHANG Zhimin ZHANG Yuhang TONG Lanxin AN Huiling CHEN Jianchun XIAO Dongguang |
| Affiliation: | 1.College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300222,China;2.Hebei Hengshui Laobaigan Liquor Co.,Ltd.,Hengshui 053000,China;3.College of Life Sience,Hengshui University,Hengshui 053000,China) |
| Abstract: | The solid-state fermentation conditions for the production of liquifying enzyme and glucoamylase by Aspergillus niger M2 were investigated.Based on single factor test,the enzyme production conditions were optimized by L9（34） orthogonal test.The optimal fermentation conditions for liquifying enzyme production were as followed： fermentation temperature 27℃,inoculum 3×106cfu/g,culture time 4.5d and the moisture 34%.Under these conditions,the enzyme activity reached 7.65U/g.The optimal culture conditions of glucoamylase production were as followed： fermentation temperature 28℃,inoculum 3×106cfu/g and culture time 3.5d and the moisture 35%.Under these conditions,the enzyme activity reached 630U/g. |
| Keywords: | Aspergilus niger liquifying enzyme glucoamylase solid state fermentation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |