| 香菇深层发酵培养条件的优化 |
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| 引用本文： | 马琼,畅雷,吴雄.香菇深层发酵培养条件的优化[J].中国酿造,2009(6). |
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| 作者姓名： | 马琼 畅雷 吴雄 |
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| 作者单位： | 1. 生物资源保护与利用湖北省重点实验室,湖北,恩施,445000;湖北民族学院,生物科学与技术学院,湖北,恩施,445000
2. 生物资源保护与利用湖北省重点实验室,湖北,恩施,445000 |
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| 基金项目： | 湖北省教育厅资助项目，湖北民族学院生物科学与技术学院资助项目 |
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| 摘 要： | 通过单因子试验，研究了装液量、培养基pH值、培养温度、培养时间及转速对香菇菌丝摇瓶发酵的影响，并通过正交试验确定了香菇深层发酵的优化条件，即采用250mL摇瓶发酵，培养基初始pH值为6，装液量100mL，培养温度26℃，转速125r／min，发酵14d。采用优化的发酵条件进行香菇菌丝摇瓶发酵，干菌丝体得率高达0．729g/100mL。
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| 关 键 词： | 香菇 发酵 培养条件 菌丝 |
Optimization of submerged culture conditions of Lentinula edodes |
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| MA Qiong,CHANG Lei,WU Xiong.Optimization of submerged culture conditions of Lentinula edodes[J].China Brewing,2009(6). |
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| Authors: | MA Qiong CHANG Lei WU Xiong |
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| Abstract: | Using single factor tests, the effects of medium volume, medium pH, culture temperature, incubation time and stir speed on hyphae yield of Lentinula edodes in-depth fermentation were studied.The optimal submerged culture conditions for Lentinula edodes was obtained by orthogonal test. The results were as follows: initial pH 6, volume 100mL, culture temperature 26℃, stir 120r/min and incubation time 14d in 250mL flask.The maximum biomass of hyphae was 0.729g/100mL by culture under the optimal fermentation conditions. |
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| Keywords: | Lentinula edodes fermentation conditions hyphae |
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