| 琼胶酶高产海洋细菌QM42的发酵条件优化 |
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| 引用本文： | 王霁宁,严孝强,杜宗军.琼胶酶高产海洋细菌QM42的发酵条件优化[J].中国酿造,2012,31(7):86-89. |
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| 作者姓名： | 王霁宁 严孝强 杜宗军 |
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| 作者单位： | 山东大学海洋学院,山东威海,264209 |
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| 基金项目： | 山东大学威海分校第六届科研立项项目（A11023） |
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| 摘 要： | 通过单因素试验和正交试验对琼胶酶高产海洋细菌QM42发酵条件进行了优化.经过发酵培养试验,确定优化培养基组成:蛋白胨浓度5.0g/L、酵母粉浓度0.50g/L、琼胶浓度3.0g/L;在培养初始pH值为7.0、装液量50mL/150mL、培养盐度50g/L,29℃条件下发酵48h,粗酶液酶活力达到627.65U/mL.
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| 关 键 词： | 琼胶酶 发酵条件 优化 正交试验 |
Optimization of fermentation conditions for agarase-producing marine bacterium QM42 |
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| WANG Jining
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YAN Xiaoqiang
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DU Zongjun.Optimization of fermentation conditions for agarase-producing marine bacterium QM42[J].China Brewing,2012,31(7):86-89. |
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| Authors: | WANG Jining YAN Xiaoqiang DU Zongjun |
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| Affiliation: | (College of Marine Science,Shandong University at Weihai,Weihai 264209,China) |
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| Abstract: | The fermentation conditions of an agarase-producing marine bacterium QM42 were optimized by single factor text and orthogonal test.Results showed that the optimal culture medium consisted of 5.0g/L peptone,0.50g/L yeast extract and 3.0g/L agar.The optimal environmental conditions were as followed： temperature 29℃,the initial pH value 7.0,the salinity 50g/L,liquid medium volume 50ml in 150ml shake flask and fermentation time 48h.Under such conditions,agarase activity reached 627.65U/ml. |
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| Keywords: | agarase fermentation conditions optimization orthogonal experiment |
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