| 放线菌327#的摇瓶补料分批发酵研究 |
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| 引用本文： | 赵子鹏,朱薇玲,王小琴,黄浩.放线菌327#的摇瓶补料分批发酵研究[J].中国酿造,2012,31(6):122-125. |
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| 作者姓名： | 赵子鹏 朱薇玲 王小琴 黄浩 |
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| 作者单位： | 1. 武汉工业学院生物与制药工程学院,湖北武汉,430023
2. 武汉工业学院生物与制药工程学院,湖北武汉430023;武汉工业学院省级生物学实验教学示范中心,湖北武汉430023 |
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| 摘 要： | 为提高放线菌327#发酵生产拮抗物质的产量,在摇瓶中采用补料分批发酵方式,考察了发酵过程中补加碳源和氮源对菌体生产拮抗物质的影响.试验结果表明:在发酵36h补加0.5％大豆粉,48h补加1.5％葡萄糖,60h补加0.5％葡萄糖的补料方式获得了最佳的补料分批发酵结果,拮抗物质产量最高,其产生的抑菌圈平均直径达(25.2±0.4) mm,比对照(22.3+0.4) mm有显著的增加.
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| 关 键 词： | 放线菌327# 补料分批发酵 拮抗物质 摇瓶 |
Fed-batch fermentation of actinomycete 327^# in shake flasks |
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| ZHAO Zipeng
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ZHU Weiling
,
WANG Xiaoqin
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HUANG Hao.Fed-batch fermentation of actinomycete 327^# in shake flasks[J].China Brewing,2012,31(6):122-125. |
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| Authors: | ZHAO Zipeng ZHU Weiling WANG Xiaoqin HUANG Hao |
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| Affiliation: | ZHAO Zipeng, ZHU Weiling, WANG Xiaoqin, HUANG Ha(l.College of Biotechnology and Pharmaceutical Engineeting, Wuhan Polytechnic University, Wuhan 430023, China; 2.Hubei Experimental Teaching Demonstration Center of Biology, Wuhan Polytechnic University, Wuhan 430023, China) |
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| Abstract: | To improve the yield of antagonistic substances produced by actinomycete 327^#, the effects of carbon or nitrogen source feeding on the pro-duction of antagonistic substances were investigated by fed-batch fermentation in shake flasks. The results indicated that feeding 0.5% soybean flour at 36h, 1.5% glucose at 48h and 0.5% glucose at 60h during the fed-batch fermentation could significantly entaance the production of antagonistic substances, whose mean inhibition zone diameter reached （25.2±0.4）mm which was significantly higher than control （22.3±0.4）mm. |
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| Keywords: | actinomycete 327^# fed-batch fermentation antagonistic substances shake flask |
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