一株耐高温解磷真菌溶磷条件的优化

从白酒丢糟高温堆肥样品中分离纯化得到一株具有耐高温能力的解磷真菌GDF1，并对该菌株的溶磷条件进行了优化。利用单因素试验，确定该菌溶磷的最适碳氮源种类及碳氮源、无机盐和磷源浓度；再通过响应面法进一步分析各因素的交互作用。结果表明，菌株GDF1最适溶磷条件为蔗糖10.7 g/L，草酸铵0.6 g/L，复合无机盐1.0 g/L，磷酸三钙10.7 g/L。在此优化条件下，其对磷酸三钙的溶解量可达292.59 mg/L，在高温条件下生物溶磷方面具有较好的应用潜力。

Optimization of phosphorus solubilization conditions for phosphate-solubilizing fungus with high-temperature resistance

A strain of phosphorus-solubilizing fungus GDF1 with high temperature resistance was isolated and purified from the high-temperature compost sample of Baijiu (Chinese liquor) distiller's grains, and the phosphorus-solubilizing conditions of the strain were optimized. Using single factor experiments, the optimal types and concentrations of carbon and nitrogen sources, concentrations of inorganic salts and phosphorus sources of the strain for phosphorus-solubilizing were determined, and the interaction of each factor was further analyzed by response surface methodology. The results shown that the optimal phosphorus-solubilizing conditions of strain GDF1 were determined as follows: sucrose 10.7 g/L, ammonium oxalate 0.6 g/L, compound inorganic salt 1.0 g/L, and tricalcium phosphate 10.7 g/L. Under the optimal conditions, the solubilizing amount of tricalcium phosphate could reach 292.59 mg/L, which had good application potential in the field of biological phosphorus-solubilizing under high temperature conditions.