RT-FQPCR法快速检测葡萄酒中醋酸菌群

建立一种快速、灵敏的检测葡萄酒中醋酸菌群的检测技术。通过改进葡萄酒中脱氧核糖核酸（DNA）提取方法，设计醋酸菌通用引物，优化实时荧光定量聚合酶链式反应（RT-FQPCR）条件，确定葡萄酒中醋酸菌的快速检测方法，同时利用7种醋酸菌对该方法的通用性、特异性以及灵敏度进行了验证。结果表明，7种醋酸菌均具有明显扩增曲线；非目的菌在该种条件下未见扩增，综合检测灵敏度为124 CFU/mL。以10种市售葡萄酒为基质，在其中添加醋酸菌均可检测出目标添加菌株。该方法通用性强，可用于葡萄酒中醋酸菌群的检测。

Rapid detection of acetic acid bacteria flora in wine by RT-FQPCR

A rapid and sensitive detection technique for acetic acid bacteria flora in wine was established by improving the extraction method of DNA in wine. The universal primers for acetic acid bacteria were designed, and the reaction conditions were optimized of real-time fluorescence quantitative polymerase chain reaction (RT-FQPCR). At the same time, the universality, specificity and sensitivity of the method were verified using 7 acetic acid bacteria strains. The results showed that 7 strains had obvious amplification curves, non-target bacteria were not amplified under this condition, and the comprehensive detection sensitivity was 124 CFU/ml. Based on 10 commercial wines, the target strains could be detected by adding acetic acid bacteria, indicating the method had high universality and could be used for detecting acetic acid bacteria flora in wine.