| 黑曲霉TNA-09中α-葡萄糖苷酶基因敲除的研究 |
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| 引用本文： | 王露,杨赢,刘燕,王亚军,杨建松,高年发,王德培.黑曲霉TNA-09中α-葡萄糖苷酶基因敲除的研究[J].中国酿造,2013(11):27-31. |
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| 作者姓名： | 王露 杨赢 刘燕 王亚军 杨建松 高年发 王德培 |
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| 作者单位： | [1]天津科技大学生物工程学院,天津300457 [2]天津市工业微生物重点实验室,天津300457 |
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| 基金项目： | 科技部高科技发展研究计划（863项目）（2011AA02A205） |
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| 摘 要： | 以柠檬酸生产菌黑曲霉TNA—09为原始菌株，通过农杆菌介导的方法，将其α-葡萄糖苷酶基因敲除，得到基因敲除菌株TGA101。对原始菌株黑曲霉TNA-09和基因敲除菌TGA101 α-葡萄糖苷酶活力测定，结果相对于TNA—09菌株，TGA101菌株α-葡萄糖苷酶活力下降61．15％。在柠檬酸发酵试验中表现出相对于黑曲霉TNA-09，TGA101菌株发酵液中异麦芽糖含量降低84．67％，柠檬酸提高2．34％，糖酸转化率提高2．34％。
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| 关 键 词： | 黑曲霉TNA-09 α-葡萄糖苷酶 异麦芽糖 基因敲除 柠檬酸 |
Alpha-glucosidase gene knockout of Aspergillus niger TNA-09 |
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| WANG Lu,YANG Ying,LILT Yan,WANG Yajun,YANG Jiansong,GAO Nianfa,WANG Depei.Alpha-glucosidase gene knockout of Aspergillus niger TNA-09[J].China Brewing,2013(11):27-31. |
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| Authors: | WANG Lu YANG Ying LILT Yan WANG Yajun YANG Jiansong GAO Nianfa WANG Depei |
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| Affiliation: | 1.College of Biotechnology, Tianjin University of Science ＆ Technology, Tianjin 300457, China; 2. Tianjin Key Laboratory of Industrial Microbiology, Tianjin 300457, China) |
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| Abstract: | In this thesis, α-glucosidase gene of the original strain Aspergillus niger TNA-09 was knockouted by Agrobacterium-mediated method in citric acid production, and knockout strain TGA101 was obtained, α-glucosidase activity of gene knockout strain TGA101 was detected, which decreased by 61.15% than original strain of A. niger TNA-09. It was showed in the citric acid fermentation experiments, compared to A. niger TNA-09, TGA 101 fermentation liquid isomaltose decreased by 84.67%, citric acid increased by 2.34%, sugar/acid conversion rate increased by 2.34%. |
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| Keywords: | Aspergillus niger TNA-09 α-glucoamylase isomaltose gene knockout citric acid |
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