| 层析法分离纯化纳豆激酶的技术参数研究 |
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| 引用本文： | 郭希娟,张桂芳,刘远洋.层析法分离纯化纳豆激酶的技术参数研究[J].中国酿造,2011(10). |
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| 作者姓名： | 郭希娟 张桂芳 刘远洋 |
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| 作者单位： | 1. 黑龙江八一农垦大学食品学院,黑龙江大庆,163319
2. 黑龙江省农产品加工工程技术研究中心,黑龙江大庆,163319 |
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| 摘 要： | 采用柱层析技术对盐析后的纳豆激酶发酵液进行分离纯化,以确定层析分离纳豆激酶的技术参数.对比了Sephadex G-50及Sephadex G-7凝胶层析分离纳豆激酶的效果,并进行了不同pH值条件下的层析效果分析.结果表明,在pH值为6.4时,经Sephadex G-75凝胶层析及SP Sepharose FF离子交换层析后,能够得到较高纯度的纳豆激酶,使纳豆激酶纯化了9.2倍,回收率为51.5％.SDS-PAGE电泳显示所得纳豆激酶为单一蛋白带,其分子质量为28ku.
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| 关 键 词： | 发酵液 纳豆激酶 分离 纯化 凝胶层析 离子交换层析 |
Separation and purification ofnattokinase by chromatography |
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| GUO Xijuan,ZHANG Guifang,LIU Yuanyang.Separation and purification ofnattokinase by chromatography[J].China Brewing,2011(10). |
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| Authors: | GUO Xijuan ZHANG Guifang LIU Yuanyang |
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| Affiliation: | GUO Xijuan1,ZHANG Guifang2,LIU Yuanyang1(1.Food College,Heilongjiang Ba Yi Land Reclamation University,Daqing 163319,China,2.Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province,China) |
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| Abstract: | In order to obtain high purity nattokinase from the fermentation broth after salting-out,some separation and purification techniques and parameters were explored.Two kinds of gel chromatography of SephadexG-50 and SephadexG-75 were compared and the conditions of separation and purification under different pH values were investigated.The results indicated that high purity nattokinase can be obtained by SephadexG-75 gel filtration and SP Sepharose FF ion-exchange chromatography under pH value 6.4.The purifica... |
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| Keywords: | fermentation broth nattokinase separation purification gel chromatography ion-exchange chromatography |
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