香菇柄双酶同步糖化工艺优化

以香菇柄为主要原料，采用纤维素酶和糖化酶双酶同步酶解工艺，通过单因素试验和Box-Behnken响应面试验优化香菇柄糖化工艺。结果表明，双酶同步糖化最佳工艺条件为：纤维素酶和糖化酶酶活比5∶3、双酶添加量3 164 U/g、料液比1∶15（g∶mL）、酶解时间120 min，酶解温度50 ℃。在此优化条件下，香菇柄水解液还原糖得率达11.05%。香菇柄双酶同步糖化条件温和、安全性高，可提高香菇柄还原糖含量，可为香菇柄无添加糖酿造酒等发酵食品的开发提供参考。

Saccharification technology optimization of Lentinus edodes stem by synchronous double enzymes hydrolysis

Using Lentinus edodes stem as main raw material, the synchronously bienzymatic saccharification technology with cellulose and glucoamylase was optimized by single factor and Box-Behnken response surface experiments. The results showed that the optimum synchronous bienzymatic saccharification conditions were obtained as follows: cellulose and glucoamylase activity ratio 5:3, compound enzymes addition 3 164 U/g, material and liquid ratio 1∶15 (g∶ml), enzymolysis time 120 min, and temperature 50 ℃. Under these optimal conditions, the yield of reducing sugar from L. edodes stem hydrolysate reached 11.05%. The synchronously bienzymatic saccharification conditions of L. edodes stem were mild, highly safe, and could increase the reducing sugar content of L. edodes stem, which could provide a reference for the development of fermented foods such as sugar-free L. edodes stem alcoholic drink.