凯里红酸汤对大鼠肠道脂肪酸吸收和紧密连接蛋白的调控

通过灌胃摄入的方式观察贵州凯里红酸汤对高脂血症大鼠模型的小肠上皮脂肪酸吸收蛋白和紧密连接蛋白的调控作用。摄入凯里红酸汤期间测量大鼠的体质量和血脂，摄入11周后测定大鼠小肠上皮的脂肪酸转位酶CD36（FAT/CD36）、脂肪酸转运蛋白（FATP4）、紧密连接相关蛋白Occludin和紧密连接跨膜蛋白ZO-1的表达。结果表明，在高脂血症大鼠模型中，酸汤干预使大鼠的体质量和血脂显著降低（P＜0.05），CD36和FATP4表达水平显著降低（P＜0.05），ZO-1表达水平显著升高（P＜0.05），其水平更接近于正常条件下喂养的阴性组大鼠。酸汤可以抑制大鼠体质量增长，恢复被高脂膳食破坏的小肠上皮紧密连接结构，减少肠道长链脂肪酸的吸收，改善大鼠高脂血症的状况。

Regulation of Kaili red sour soup on fatty acid absorption and tight junction protein in rat intestine

The regulation of Kaili red sour soup on the fatty acid absorption protein and tight junction protein of small intestinal epithelium in hyperlipidemic rat model was observed by gavage. The weight and blood lipid of the rats were measured during the intake of Kaili red sour soup, and the expression of fatty acid translocase (FAT/CD36), fatty acid transport protein (FATP4), tight junction related protein Occludin and tight junction transmembrane protein ZO-1 of the small intestinal epithelium of rat were measured after 11 weeks of intake. The results showed that in the hyperlipidemic rat model, the intervention of sour soup significantly reduced the body mass and blood lipid of rats (P＜0.05), the expression levels of CD36 and FATP4 significantly decreased (P＜0.05), the expression level of ZO-1 significantly increased (P＜0.05), and its level was closer to that of the rats fed under normal conditions in negative group. The sour soup could inhibit the increase of rat body mass, restore the tight junction structure of the small intestine epithelium destroyed by the high-fat diet, reduce the absorption of long-chain fatty acids in the intestine, and improve the hyperlipidemia condition.